author = { Vaidehi, Nagarajan },

title = { Membrane protein structure and dynamics methods and protocols },

publisher = {Humana Press},

isbn = {9781627030229},

keywords = { Membrane Proteins Laboratory Manuals , Membrane Proteins physiology Laboratory Manuals , Membrane Proteins ultrastructure Laboratory Manuals , Membranproteine },

year = {2012},

abstract = {Literaturangaben},

abstract = {Crystallization of membrane proteins in bicelles -- Vapor diffusion-controlled meso crystallization of membrane proteins -- Solution NMR studies of integral polytopic alpha-helical membrane proteins: The structure determination of the seven-helix transmembrane receptor sensory rhodopsin ii, psrii -- Use of NMR saturation transfer difference spectroscopy to study ligand binding to membrane proteins -- How to investigate interactions between membrane proteins and ligands by solid-state NMR -- Identifying and measuring transmembrane helix-helix interactions by FRET -- Studying substrate binding to reconstituted secondary transporters by attenuated total reflection infrared difference spectroscopy -- UV-visible and infrared methods for investigating lipid-rhodopsin membrane interactions -- Proteomic characterization of integral membrane proteins using thermostatted liquid chromatography coupled with tandem mass spectrometry -- LITiCon: A discrete conformational sampling computational method for mapping various functionally selective conformational states of transmembrane helical proteins -- Homology model-assisted elucidation of binding sites in GPCRs -- Comparative modeling of lipid receptors -- Quantification of structural distortions in the transmembrane helices of GPCRs -- Structure prediction of g protein-coupled receptors and their ensemble of functionally important conformations -- Target based virtual screening by docking into automatically generated GPCR models -- Predicting the biological activities through QSAR analysis and docking-based scoring -- Identification of motions in membrane proteins by elastic network models and their experimental validation -- Modeling the structural communication in supramolecular complexes involving GPCRs -- Exploring substrate diffusion in channels using biased molecular dynamics simulations.},

booktitle = {Methods in molecular biology ; 914},

booktitle = {Springer protocols},

address = { New York },

url = { http://slubdd.de/katalog?TN_libero_mab2 }