> Details

Marx-Blümel, Lisa [Author]; Marx, Christian [Author]; Sonnemann, Jürgen [Author]; Weise, Frank [Author]; Hampl, Jörg [Author]; Frey, Jessica [Author]; Rothenburger, Linda [Author]; Cirri, Emilio [Author]; Rahnis, Norman [Author]; Koch, Philipp [Author]; Groth, Marco [Author]; Schober, Andreas [Author]; Wang, Zhao-Qi [Author]; Beck, James F. [Author]

Molecular characterization of hematopoietic stem cells after in vitro amplification on biomimetic 3D PDMS cell culture scaffolds

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Molecular characterization of hematopoietic stem cells after in vitro amplification on biomimetic 3D PDMS cell culture scaffolds
  • Contributor: Marx-Blümel, Lisa [VerfasserIn]; Marx, Christian [VerfasserIn]; Sonnemann, Jürgen [VerfasserIn]; Weise, Frank [VerfasserIn]; Hampl, Jörg [VerfasserIn]; Frey, Jessica [VerfasserIn]; Rothenburger, Linda [VerfasserIn]; Cirri, Emilio [VerfasserIn]; Rahnis, Norman [VerfasserIn]; Koch, Philipp [VerfasserIn]; Groth, Marco [VerfasserIn]; Schober, Andreas [VerfasserIn]; Wang, Zhao-Qi [VerfasserIn]; Beck, James F. [VerfasserIn]
  • imprint: 2021
  • Published in: Scientific reports ; 11(2021) vom: 27. Okt., Artikel-ID 21163, Seite 1-14
  • Language: English
  • DOI: 10.1038/s41598-021-00619-6
  • ISSN: 2045-2322
  • Identifier:
  • Origination:
  • Footnote:
  • Description: Hematopoietic stem cell (HSC) transplantation is successfully applied since the late 1950s. However, its efficacy can be impaired by insufficient numbers of donor HSCs. A promising strategy to overcome this hurdle is the use of an advanced ex vivo culture system that supports the proliferation and, at the same time, maintains the pluripotency of HSCs. Therefore, we have developed artificial 3D bone marrow-like scaffolds made of polydimethylsiloxane (PDMS) that model the natural HSC niche in vitro. These 3D PDMS scaffolds in combination with an optimized HSC culture medium allow the amplification of high numbers of undifferentiated HSCs. After 14 days in vitro cell culture, we performed transcriptome and proteome analysis. Ingenuity pathway analysis indicated that the 3D PDMS cell culture scaffolds altered PI3K/AKT/mTOR pathways and activated SREBP, HIF1α and FOXO signaling, leading to metabolic adaptations, as judged by ELISA, Western blot and metabolic flux analysis. These molecular signaling pathways can promote the expansion of HSCs and are involved in the maintenance of their pluripotency. Thus, we have shown that the 3D PDMS scaffolds activate key molecular signaling pathways to amplify the numbers of undifferentiated HSCs ex vivo effectively.
  • Access State: Open Access
  • Rights information: Attribution (CC BY)