• Media type: Book
  • Title: Drosophila : methods and protocols
  • Contributor: Dahmann, Christian [HerausgeberIn]
  • Published: New York: Humana Press, [2016]
  • Published in: Methods in molecular biology ; 1478
    Springer protocols
  • Issue: Second edition
  • Contains: Using FlyBase, a database of Drosophila genes and genomes / Steven J. Marygold [and others]
    Using FlyBase, a database of Drosophila genes and genomes / Steven J. Marygold ... [et al.]
    GAL4 system : a versatile system for the manipulation and analysis of gene expression / Elizabeth E. Caygill and Andrea H. Brand
    Q-System : a versatile expression system for Drosophila / Olena Riabinina and Christopher J. Potter
    Analysis of microRNA runction in Drosophila / Geetanjali Chawla, Arthur Luhur, and Nicholas Sokol
    Methods for high-throughput RNAi screening in Drosophila cells / Maximilian Billmann and Michael Boutros
    Guide to genome-wide in vivo RNAi applications in Drosophila / Aynur Kaya-Çopur and Frank Schnorrer
    Creating heritable mutations in Drosophila with CRISPR-Cas9 / Fillip Port and Simon L. Bullock
    Performing chromophore-assisted laser inactivation in Drosophila embryos using GFP / Anne Pélissier-Monier, Bénédicte Sanson, and Bruno Monier
    deGradFP : a system to knockdown GFP-tagged proteins / Emmanuel Caussinus and Markus Affolter
    Sample preparation and mounting of Drosophila embryos for multiview light sheet microscopy / Christopher Schmied and Pavel Tomancak
    Cultivation and live imaging of Drosophila imaginal discs / Simon Restrepo, Jeremiah J. Zartman, and Konrad Basler
    Cultivation and live imaging of Drosophila ovaries / Maureen Cetera, Lindsay Lewellyn, and Sally Horne-Badovinac
    Segmentation and quantitative analysis of epithelial tissues / Benoit Aigouy, Daiki Umetsu, and Suzanne Eaton
    Laser ablation to probe the epithelial mechanics in Drosophila / Pruthvi C. Shivakumar and Pierre-Francois Lenne
    Rapid ovary mass-isolation (ROMi) to obtain large quantities of Drosophila egg chambers for fluorescent in situ hybridization / Helena Jambor, Pavel Mejstrik, and Pavel Tomancak
    Chromatin immunoprecipitation for analyzing transcription factor binding and histone modifications in Drosophila / Yad Ghavi-Helm, Bingqing Zhao, and Eileen E.M. Furlong
    Protocols to study growth and metabolism in Drosophila / Katrin Strassburger and Aurelio A. Teleman
    Protocols to study aging in Drosophila / Matthew D.W. Piper and Linda Partridge
    Protocols to study behavior in Drosophila / Wendi S. Neckameyer and Parag Bhatt
    Electrophysiological recordings from lobula plate tangential cells in Drosophila / Alex S. Mauss and Alexander Borst
    Methods to establish Drosophila cell lines / Alain Debec, Timothy L. Megraw, and Antoine Guichet.
    GAL4 system : a versatile system for the manipulation and analysis of gene expression / Elizabeth E. Caygill and Andrea H. Brand
    Q-System : a versatile expression system for Drosophila / Olena Riabinina and Christopher J. Potter
    Analysis of microRNA runction in Drosophila / Geetanjali Chawla, Arthur Luhur, and Nicholas Sokol
    Methods for high-throughput RNAi screening in Drosophila cells / Maximilian Billmann and Michael Boutros
    Guide to genome-wide in vivo RNAi applications in Drosophila / Aynur Kaya-Çopur and Frank Schnorrer Creating heritable mutations in Drosophila with CRISPR-Cas9
    Performing chromophore-assisted laser inactivation in Drosophila embryos using GFP / Anne Pélissier-Monier, Bénédicte Sanson, and Bruno Monier
    deGradFP : a system to knockdown GFP-tagged proteins / Emmanuel Caussinus and Markus Affolter
    Sample preparation and mounting of Drosophila embryos for multiview light sheet microscopy / Christopher Schmied and Pavel Tomancak
    Cultivation and live imaging of Drosophila imaginal discs / Simon Restrepo, Jeremiah J. Zartman, and Konrad Basler
    Cultivation and live imaging of Drosophila ovaries / Maureen Cetera, Lindsay Lewellyn, and Sally Horne-Badovinac
    Segmentation and quantitative analysis of epithelial tissues / Benoit Aigouy, Daiki Umetsu, and Suzanne Eaton
    Laser ablation to probe the epithelial mechanics in Drosophila / Pruthvi C. Shivakumar and Pierre-Francois Lenne
    Rapid ovary mass-isolation (ROMi) to obtain large quantities of Drosophila egg chambers for fluorescent in situ hybridization / Helena Jambor, Pavel Mejstrik, and Pavel Tomancak
    Chromatin immunoprecipitation for analyzing transcription factor binding and histone modifications in Drosophila / Yad Ghavi-Helm, Bingqing Zhao, and Eileen E. M. Furlong
    Protocols to study growth and metabolism in Drosophila / Katrin Strassburger and Aurelio A. Teleman
    Protocols to study aging in Drosophila / Matthew D. W. Piper and Linda Partridge
    Protocols to study behavior in Drosophila / Wendi S. Neckameyer and Parag Bhatt
    Electrophysiological recordings from lobula plate tangential cells in Drosophila / Alex S. Mauss and Alexander Borst
    Methods to establish Drosophila cell lines / Alain Debec, Timothy L. Megraw, and Antoine Guichet.
  • Extent: xii, 355 Seiten; Illustrationen
  • Language: English
  • ISBN: 9781493963690
  • Origination:
  • RVK notation: WC 4460 : Molekulargenetische Methoden: DNS-Analyse, Klonierung, Mutagenese etc.
    WC 6340 : Insekten
    WC 4150 : Allgemeines, Praktika; "Methods of biochemical analysis"
  • Keywords: Drosophila
  • Description: "This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls"--
  • Footnote: Includes bibliographical references and index
  • Acqusition status: ordered