You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Intravital 2-photon imaging reveals distinct morphology and infiltrative properties of glioblastoma-associated macrophages
Contributor:
Chen, Zhihong;
Ross, James L.;
Hambardzumyan, Dolores
Published:
Proceedings of the National Academy of Sciences, 2019
Published in:
Proceedings of the National Academy of Sciences, 116 (2019) 28, Seite 14254-14259
Language:
English
DOI:
10.1073/pnas.1902366116
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
Significance Therapeutic strategies targeting glioblastoma (GBM) tumor-associated macrophages (TAMs) have shown limited efficacy. One reason for this limited effectiveness is our poor understanding of the fundamental characteristics driving the behavior of these cells. Here we report a combination of immunocompetent lineage tracing mouse models of GBM with an open skull window for in vivo observation of TAM constituents via 2-photon microscopy. We show that TAMs are composed of 2 morphologically and behaviorally distinct cell types: brain-resident microglia and bone marrow-derived macrophages (BMDMs). After treatment with anti-vascular endothelial growth factor A antibody, we observed significant reductions in BMDM infiltration and morphological responses. These studies provide a platform for monitoring the multidimensional responses of specific cell populations to therapeutic intervention in real time.