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Media type:
E-Article
Title:
Using translational enhancers to increase transgene expression in Drosophila
Contributor:
Pfeiffer, Barret D.;
Truman, James W.;
Rubin, Gerald M.
Published:
Proceedings of the National Academy of Sciences, 2012
Published in:
Proceedings of the National Academy of Sciences, 109 (2012) 17, Seite 6626-6631
Language:
English
DOI:
10.1073/pnas.1204520109
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
The ability to specify the expression levels of exogenous genes inserted in the genomes of transgenic animals is critical for the success of a wide variety of experimental manipulations. Protein production can be regulated at the level of transcription, mRNA transport, mRNA half-life, or translation efficiency. In this report, we show that several well-characterized sequence elements derived from plant and insect viruses are able to function in Drosophila to increase the apparent translational efficiency of mRNAs by as much as 20-fold. These increases render expression levels sufficient for genetic constructs previously requiring multiple copies to be effective in single copy, including constructs expressing the temperature-sensitive inactivator of neuronal function Shibire ts1 , and for the use of cytoplasmic GFP to image the fine processes of neurons.