> Details

Bechar, J; Bastie, C; Dallmann, R; Worch, J; Gharanei, S; Shabir, S; Dove, A; Hardwicke, J

O054 A novel hyaluronic acid hydrogel as an extracellular matrix to improve adipose tissue graft survival in autologous transfer for breast cancer reconstruction

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: O054 A novel hyaluronic acid hydrogel as an extracellular matrix to improve adipose tissue graft survival in autologous transfer for breast cancer reconstruction
  • Contributor: Bechar, J; Bastie, C; Dallmann, R; Worch, J; Gharanei, S; Shabir, S; Dove, A; Hardwicke, J
  • Published: Oxford University Press (OUP), 2022
  • Published in: British Journal of Surgery, 109 (2022) Supplement_4
  • Language: English
  • DOI: 10.1093/bjs/znac242.054
  • ISSN: 0007-1323; 1365-2168
  • Keywords: Surgery
  • Origination:
  • Footnote:
  • Description: Abstract Introduction Adipose tissue transfer is used for correcting breast contour defects after cancer surgery. Graft survival can be as poor as 15% at four years follow-up. Hyaluronic acid (HA) hydrogels can be used to suspend adipocytes to aid survival. Methods A unique HA gel with cross-linked aldehyde and hydrazine groups allowing self-healing around cells after injection was engineered. The gel's rheology and degradation properties in vitro were characterised. 3T3-L1, murine and human adipocytes were encapsulated within HA hydrogel, with survival and gene expression interrogated. A murine animal model underwent subcutaneous injection of primary murine adipocytes mixed with HA gel, with survival and gene expression examined. Results The novel HA hydrogel can be degraded by tissue hyaluronidases in 4–5 days. Self-healing occurred in 30 minutes and the gel is robust enough to be injected through a needle. 3T3-L1, murine and human adipocytes were encapsulated within HA hydrogel and showed a <15% deduction in survival compared to control. Mature adipocyte gene expression markers compared to control showed no difference. Primary murine adipocytes injected in vivo showed no difference in survival at higher gel volumes compared to control. Conclusion The novel engineered HA hydrogel has suitable mechanical characteristics for injection and can support adipocyte survival in vitro and in vivo and is ready for a pilot human trial. Take-home message Our novel HA gel can support adipocytes in vivo and in vitro, and offers a potential method for augmentation of adipose tissue transfer.