Description:
<jats:title>Abstract</jats:title><jats:p>The optimal separation conditions and online sample concentration for <jats:italic>N</jats:italic>,<jats:italic>N</jats:italic>‐dimethyltryptamine (DMT) and related compounds, including α‐methyltryptamine (AMT), 5‐methoxy‐AMT (5‐MeO‐AMT),<jats:italic> N</jats:italic>,<jats:italic>N</jats:italic>‐diethyltryptamine (DET), <jats:italic>N</jats:italic>,<jats:italic>N</jats:italic>‐dipropyltryptamine (DPT),<jats:italic> N</jats:italic>,<jats:italic>N</jats:italic>‐dibutyltryptamine (DBT), <jats:italic>N</jats:italic>,<jats:italic>N</jats:italic>‐diisopropyltryptamine (DiPT), 5‐methoxy‐DMT (5‐MeO‐DMT), and 5‐methoxy‐<jats:italic>N</jats:italic>,<jats:italic>N</jats:italic>‐DiPT (5‐MeO‐DiPT), using micellar EKC (MEKC) with UV‐absorbance detection are described. The LODs (S/N = 3) for MEKC ranged from 1.0 ˜ 1.8 μg/mL. Use of online sample concentration methods, including sweeping‐MEKC and cation‐selective exhaustive injection‐sweep‐MEKC (CSEI‐sweep‐MEKC) improved the LODs to 2.2 ˜ 8.0 ng/mL and 1.3 ˜ 2.7 ng/mL, respectively. In addition, the order of migration of the nine tryptamines was investigated. A urine sample, obtained by spiking urine collected from a human volunteer with DMT, was also successfully examined.</jats:p>