• Media type: E-Article
  • Title: Next‐generation sequencing of PTEN mutations for monitoring minimal residual disease in T‐cell acute lymphoblastic leukemia
  • Contributor: Germano, Giuseppe; Valsecchi, Maria Grazia; Buldini, Barbara; Cazzaniga, Giovanni; Zanon, Carlo; Silvestri, Daniela; te Kronnie, Geertruij; Basso, Giuseppe; Paganin, Maddalena
  • Published: Wiley, 2020
  • Published in: Pediatric Blood & Cancer, 67 (2020) 1
  • Language: English
  • DOI: 10.1002/pbc.28025
  • ISSN: 1545-5009; 1545-5017
  • Keywords: Oncology ; Hematology ; Pediatrics, Perinatology and Child Health
  • Origination:
  • Footnote:
  • Description: AbstractMinimal residual disease (MRD) analysis has become a powerful indicator to refine therapy in acute lymphoblastic leukemia (ALL). Here, we present an MRD detection based on the next‐generation sequencing of PTEN exon 7 mutations (NGS‐PTEN) in 30 pediatric T‐cell ALL patients. By comparing the NGS‐PTEN results with current quantitative PCR of antigen receptor gene rearrangements (qPCR‐Ig/TR), an overall concordance of 80% was found between the two methods. However, the NGS‐PTEN qualified a lower number of high‐risk patients than qPCR‐Ig/TR. These findings suggest that NGS‐PTEN is a promising tool that could potentially be used to support current MRD methodologies for T‐ALL patients.