• Media type: E-Article
  • Title: Bioavailability, metabolism, and excretion of a complex Alternaria culture extract versus altertoxin II: a comparative study in rats
  • Contributor: Puntscher, Hannes; Aichinger, Georg; Grabher, Stephanie; Attakpah, Eva; Krüger, Franziska; Tillmann, Katharina; Motschnig, Tomas; Hohenbichler, Julia; Braun, Dominik; Plasenzotti, Roberto; Pahlke, Gudrun; Höger, Harald; Marko, Doris; Warth, Benedikt
  • Published: Springer Science and Business Media LLC, 2019
  • Published in: Archives of Toxicology, 93 (2019) 11, Seite 3153-3167
  • Language: English
  • DOI: 10.1007/s00204-019-02575-7
  • ISSN: 0340-5761; 1432-0738
  • Origination:
  • Footnote:
  • Description: Abstract Despite the frequent infection of agricultural crops by Alternaria spp., their toxic secondary metabolites and potential food contaminants lack comprehensive metabolic characterization. In this study, we investigated their bioavailability, metabolism, and excretion in vivo. A complex Alternaria culture extract (50 mg/kg body weight) containing 11 known toxins and the isolated lead toxin altertoxin II (0.7 mg/kg body weight) were administered per gavage to groups of 14 Sprague Dawley rats each. After 3 h and 24 h, plasma, urine and feces were collected to determine toxin recoveries. For reliable quantitation, an LC–MS/MS method for the simultaneous detection of 20 Alternaria toxins and metabolites was developed and optimized for either biological matrix. The obtained results demonstrated efficient excretion of alternariol (AOH) and its monomethyl ether (AME) via feces (> 89%) and urine (> 2.6%) after 24 h, while the majority of tenuazonic acid was recovered in urine (20 and 87% after 3 and 24 h, respectively). Moreover, modified forms of AOH and AME were identified in urine and fecal samples confirming both, mammalian phase-I (4-hydroxy-AOH) and phase-II (sulfates) biotransformation in vivo. Despite the comparably high doses, perylene quinones were recovered only at very low levels (altertoxin I, alterperylenol, < 0.06% in urine and plasma, < 5% in feces) or not at all (highly genotoxic, epoxide-holding altertoxin II, stemphyltoxin III). Interestingly, altertoxin I was detected in all matrices of rats receiving altertoxin II and suggests enzymatic de-epoxidation in vivo. In conclusion, the present study contributes valuable information to advance our understanding of the emerging Alternaria mycotoxins and their relevance on food safety.