Braun, Thomas;
Buschhausen-Denker, Gregor;
Tannich, Egbert;
Arnold, Hans H.
The interaction of nuclear proteins with essential promoter element of the chicken cardiac myosin light chain 2 gene is involved in muscle‐specific transcription
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Media type:
E-Article
Title:
The interaction of nuclear proteins with essential promoter element of the chicken cardiac myosin light chain 2 gene is involved in muscle‐specific transcription
Contributor:
Braun, Thomas;
Buschhausen-Denker, Gregor;
Tannich, Egbert;
Arnold, Hans H.
Description:
<jats:p>A quantitative microinjection procedure has been developed to demonstrate muscle‐specific transcription of the myosin light chain 2‐A (MLC2‐A) promoter in differentiated chicken primary breast muscle cells. Nuclear protein binds to the distal region of the required promoter sequence but not to a mutated version of this sequence. The functional significance of this specific DNA‐protein interaction for the promoter activity is demonstrated by ‘in vivo’ competition of microinjected MLC‐CAT reporter construct together with excess of synthetic oligonucleotides encompassing the protein binding sites.</jats:p>