Marsilio, Sina;
Newman, Shelley J.;
Estep, James Scot;
Giaretta, Paula R.;
Lidbury, Jonathan A.;
Warry, Emma;
Flory, Andi;
Morley, Paul S.;
Smoot, Katy;
Seeley, Erin H.;
Powell, Matthew J.;
Suchodolski, Jan S.;
Steiner, Jörg M.
Differentiation of lymphocytic‐plasmacytic enteropathy and small cell lymphoma in cats using histology‐guided mass spectrometry
Description:
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Differentiation of lymphocytic‐plasmacytic enteropathy (LPE) from small cell lymphoma (SCL) in cats can be challenging.</jats:p></jats:sec><jats:sec><jats:title>Hypothesis/Objective</jats:title><jats:p>Histology‐guided mass spectrometry (HGMS) is a suitable method for the differentiation of LPE from SCL in cats.</jats:p></jats:sec><jats:sec><jats:title>Animals</jats:title><jats:p>Forty‐one cats with LPE and 52 cats with SCL.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>This is a retrospective clinicopathologic study. Duodenal tissue samples of 17 cats with LPE and 22 cats with SCL were subjected to HGMS, and the acquired data were used to develop a linear discriminate analysis (LDA) machine learning algorithm. The algorithm was subsequently validated using a separate set of 24 cats with LPE and 30 cats with SCL. Cases were classified as LPE or SCL based on a consensus by an expert panel consisting of 5‐7 board‐certified veterinary specialists. Histopathology, immunohistochemistry, and clonality testing were available for all cats. The panel consensus classification served as a reference for the calculation of test performance parameters.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Relative sensitivity, specificity, and accuracy of HGMS were 86.7% (95% confidence interval [CI]: 74.5%‐98.8%), 91.7% (95% CI: 80.6%‐100%), and 88.9% (95% CI: 80.5%‐97.3%), respectively. Comparatively, the clonality testing had a sensitivity, specificity, and accuracy of 85.7% (95% CI: 72.8%‐98.7%), 33.3% (95% CI: 14.5%‐52.2%), and 61.5% (95% CI: 48.3%‐74.8%) relative to the panel decision.</jats:p></jats:sec><jats:sec><jats:title>Conclusions and Clinical Importance</jats:title><jats:p>Histology‐guided mass spectrometry was a reliable technique for the differentiation of LPE from SCL in duodenal formalin‐fixed paraffin‐embedded samples of cats and might have advantages over tests currently considered state of the art.</jats:p></jats:sec>