Laguens, Rubén P.;
Lazarowski, Alberto J.;
Cuniberti, Luis A.;
Janavel, Gustavo L. Vera;
Cabeza Meckert, Patricia M.;
Yannarelli, Gustavo G.;
del Valle, Héctor F.;
Lascano, Elena C.;
Negroni, Jorge A.;
Crottogini, Alberto J.
Expression of the MDR-1 Gene-encoded P-glycoprotein in Cardiomyocytes of Conscious Sheep Undergoing Acute Myocardial Ischemia Followed by Reperfusion
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Media type:
E-Article
Title:
Expression of the MDR-1 Gene-encoded P-glycoprotein in Cardiomyocytes of Conscious Sheep Undergoing Acute Myocardial Ischemia Followed by Reperfusion
Contributor:
Laguens, Rubén P.;
Lazarowski, Alberto J.;
Cuniberti, Luis A.;
Janavel, Gustavo L. Vera;
Cabeza Meckert, Patricia M.;
Yannarelli, Gustavo G.;
del Valle, Héctor F.;
Lascano, Elena C.;
Negroni, Jorge A.;
Crottogini, Alberto J.
imprint:
SAGE Publications, 2007
Published in:Journal of Histochemistry & Cytochemistry
Description:
<jats:p> We have recently reported that in chronic myocardial ischemia, adult mammalian cardiomyocytes express P-glycoprotein (P-gp). We now investigate if P-gp is also expressed in acute regional ischemia followed by reperfusion. Adult conscious sheep underwent 12-min occlusion of the mid-left anterior descending artery (inflatable cuff). Successful ischemia-reperfusion was confirmed by monitoring percent systolic left ventricular anterior wall thickening (sonomicrometry) during the whole is chemic period and every 10 min over 2 hr following cuff deflation. At 3, 24, and 48 hr after reperfusion, P-gp expression was investigated by immunohistochemistry and Western blot and MDR-1 mRNA by RT-PCR. Cardiomyocytes in the occluded artery territory (but not those in remote areas) consistently expressed P-gp at their sarcolemma. Whereas at 3 and 24 hr P-gp was mainly observed in the T tubules, at 48 hr it predominated in intercalated discs and gap junctions. RT-PCR and Western blot revealed higher expression in ischemic than in control myocardium. We conclude that in adult sheep with acute myocardial ischemia, the MDR-1 gene-encoded P-gp is expressed at the sarcolemma of the cardiomyocytes from 3 hr up to at least 48 hr after reperfusion. </jats:p>