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Heide, Janna; Schulte, Sophia; Kohsar, Matin; Brehm, Thomas Theo; Herrmann, Marissa; Karsten, Hendrik; Marget, Matthias; Peine, Sven; Johansson, Alexandra M.; Sette, Alessandro; Lütgehetmann, Marc; Kwok, William W.; Sidney, John; Schulze zur Wiesch, Julian

Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19

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  • Media type: E-Article
  • Title: Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19
  • Contributor: Heide, Janna; Schulte, Sophia; Kohsar, Matin; Brehm, Thomas Theo; Herrmann, Marissa; Karsten, Hendrik; Marget, Matthias; Peine, Sven; Johansson, Alexandra M.; Sette, Alessandro; Lütgehetmann, Marc; Kwok, William W.; Sidney, John; Schulze zur Wiesch, Julian
  • Published: Public Library of Science (PLoS), 2021
  • Published in: PLOS Pathogens, 17 (2021) 9, Seite e1009842
  • Language: English
  • DOI: 10.1371/journal.ppat.1009842
  • ISSN: 1553-7374
  • Origination:
  • Footnote:
  • Description: The aim of this study was to define the breadth and specificity of dominant SARS-CoV-2-specific T cell epitopes using a comprehensive set of 135 overlapping 15-mer peptides covering the SARS-CoV-2 envelope (E), membrane (M) and nucleoprotein (N) in a cohort of 34 individuals with acute (n = 10) and resolved (n = 24) COVID-19. Following short-term virus-specific in vitro cultivation, the single peptide-specific CD4+ T cell response of each patient was screened using enzyme linked immuno spot assay (ELISpot) and confirmed by single-peptide intracellular cytokine staining (ICS) for interferon-γ (IFN-γ) production. 97% (n = 33) of patients elicited one or more N, M or E-specific CD4+ T cell responses and each patient targeted on average 21.7 (range 0–79) peptide specificities. Overall, we identified 10 N, M or E-specific peptides that showed a response frequency of more than 36% and five of them showed high binding affinity to multiple HLA class II binders in subsequent in vitro HLA binding assays. Three peptides elicited CD4+ T cell responses in more than 55% of all patients, namely Mem_P30 (aa146-160), Mem_P36 (aa176-190), both located within the M protein, and Ncl_P18 (aa86-100) located within the N protein. These peptides were further defined in terms of length and HLA restriction. Based on this epitope and restriction data we developed a novel DRB*11 tetramer (Mem_aa145-164) and examined the ex vivo phenotype of SARS-CoV-2-specific CD4+ T cells in one patient. This detailed characterization of single T cell peptide responses demonstrates that SARS-CoV-2 infection universally primes a broad T cell response directed against multiple specificities located within the N, M and E structural protein.
  • Access State: Open Access