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Piacenza, Francesco;
Cherubini, Antonio;
Galeazzi, Roberta;
Cardelli, Maurizio;
Giacconi, Robertina;
Pierpaoli, Elisa;
Marchegiani, Francesca;
Marcheselli, Fiorella;
Recchioni, Rina;
Casoli, Tiziana;
Farnocchia, Elisabetta;
Bartozzi, Beatrice;
Giorgetti, Belinda;
Stripoli, Pierpaolo;
Bonfigli, Anna Rita;
Fedecostante, Massimiliano;
Salvi, Fabio;
Pansoni, Adolfo;
Provinciali, Mauro;
Lattanzio, Fabrizia
Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department
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- Media type: E-Article
- Title: Sensibility and Specificity of the VitaPCR™ SARS-CoV-2 Assay for the Rapid Diagnosis of COVID-19 in Older Adults in the Emergency Department
- Contributor: Piacenza, Francesco; Cherubini, Antonio; Galeazzi, Roberta; Cardelli, Maurizio; Giacconi, Robertina; Pierpaoli, Elisa; Marchegiani, Francesca; Marcheselli, Fiorella; Recchioni, Rina; Casoli, Tiziana; Farnocchia, Elisabetta; Bartozzi, Beatrice; Giorgetti, Belinda; Stripoli, Pierpaolo; Bonfigli, Anna Rita; Fedecostante, Massimiliano; Salvi, Fabio; Pansoni, Adolfo; Provinciali, Mauro; Lattanzio, Fabrizia
- Published: MDPI AG, 2023
- Published in: Viruses
- Extent: 189
- Language: English
- DOI: 10.3390/v15010189
- ISSN: 1999-4915
- Keywords: Virology ; Infectious Diseases
- Abstract: <jats:p>(1) Background: During the COVID-19 pandemic, rapid and reliable diagnostic tools are needed for detecting SARS-CoV-2 infection in urgent cases at admission to the hospital. We aimed to assess the performances of the rapid molecular VitaPCR™ test (Menarini Diagnostics) in a sample of older adults admitted to the Emergency Department of two Italian hospitals (2) Methods: The comparison between the rapid VitaPCR™ and the RT-PCR was performed in 1695 samples. Two naso-pharyngeal swab samplings from each individual were obtained and processed using the VitaPCR™ and the RT-PCR for the detection of SARS-CoV-2 (3) Results: VitaPCR™ exhibited good precision (<3% CV) and an almost perfect overall agreement (Cohen’s K = 0.90) with the RT-PCR. The limit of detection of the VitaPCR™ was 4.1 copies/µL. Compared to the RT-PCR, the sensitivity, the specificity, and the positive and negative predictive values of VitaPCR™ were 83.4%, 99.9%, 99.2% and 98.3%, respectively (4) Conclusions: The VitaPCR™ showed similar sensitivity and specificity to other molecular-based rapid tests. This study suggests that the VitaPCR™ can allow the rapid management of patients within the Emergency Department. Nevertheless, it is advisable to obtain a negative result by a RT-PCR assay before admitting a patient to a regular ward.</jats:p>
- Description: <jats:p>(1) Background: During the COVID-19 pandemic, rapid and reliable diagnostic tools are needed for detecting SARS-CoV-2 infection in urgent cases at admission to the hospital. We aimed to assess the performances of the rapid molecular VitaPCR™ test (Menarini Diagnostics) in a sample of older adults admitted to the Emergency Department of two Italian hospitals (2) Methods: The comparison between the rapid VitaPCR™ and the RT-PCR was performed in 1695 samples. Two naso-pharyngeal swab samplings from each individual were obtained and processed using the VitaPCR™ and the RT-PCR for the detection of SARS-CoV-2 (3) Results: VitaPCR™ exhibited good precision (<3% CV) and an almost perfect overall agreement (Cohen’s K = 0.90) with the RT-PCR. The limit of detection of the VitaPCR™ was 4.1 copies/µL. Compared to the RT-PCR, the sensitivity, the specificity, and the positive and negative predictive values of VitaPCR™ were 83.4%, 99.9%, 99.2% and 98.3%, respectively (4) Conclusions: The VitaPCR™ showed similar sensitivity and specificity to other molecular-based rapid tests. This study suggests that the VitaPCR™ can allow the rapid management of patients within the Emergency Department. Nevertheless, it is advisable to obtain a negative result by a RT-PCR assay before admitting a patient to a regular ward.</jats:p>
- Footnote:
- Access State: Open Access