• Media type: E-Article
  • Title: Quercetin Exerts Age-Dependent Beneficial Effects on Blood Pressure and Vascular Function, But Is Inefficient in Preventing Myocardial Ischemia-Reperfusion Injury in Zucker Diabetic Fatty Rats
  • Contributor: Ferenczyova, Kristina; Kalocayova, Barbora; Kindernay, Lucia; Jelemensky, Marek; Balis, Peter; Berenyiova, Andrea; Zemancikova, Anna; Farkasova, Veronika; Sykora, Matus; Tothova, Lubomira; Jasenovec, Tomas; Radosinska, Jana; Torok, Jozef; Cacanyiova, Sona; Barancik, Miroslav; Bartekova, Monika
  • imprint: MDPI AG, 2020
  • Published in: Molecules
  • Language: English
  • DOI: 10.3390/molecules25010187
  • ISSN: 1420-3049
  • Origination:
  • Footnote:
  • Description: <jats:p>Background: Quercetin (QCT) was shown to exert beneficial cardiovascular effects in young healthy animals. The aim of the present study was to determine cardiovascular benefits of QCT in older, 6-month and 1-year-old Zucker diabetic fatty (ZDF) rats (model of type 2 diabetes). Methods: Lean (fa/+) and obese (fa/fa) ZDF rats of both ages were treated with QCT for 6 weeks (20 mg/kg/day). Isolated hearts were exposed to ischemia-reperfusion (I/R) injury (30 min/2 h). Endothelium-dependent vascular relaxation was measured in isolated aortas. Expression of selected proteins in heart tissue was detected by Western blotting. Results: QCT reduced systolic blood pressure in both lean and obese 6-month-old rats but had no effect in 1-year-old rats. Diabetes worsened vascular relaxation in both ages. QCT improved vascular relaxation in 6-month-old but worsened in 1-year-old obese rats and had no impact in lean controls of both ages. QCT did not exert cardioprotective effects against I/R injury and even worsened post-ischemic recovery in 1-year-old hearts. QCT up-regulated expression of eNOS in younger and PKCε expression in older rats but did not activate whole PI3K/Akt pathway. Conclusions: QCT might be beneficial for vascular function in diabetes type 2; however, increasing age and/or progression of diabetes may confound its vasculoprotective effects. QCT seems to be inefficient in preventing myocardial I/R injury in type 2 diabetes and/or higher age. Impaired activation of PI3K/Akt kinase pathway might be, at least in part, responsible for failing cardioprotection in these subjects.</jats:p>
  • Access State: Open Access