• Medientyp: E-Book; Hochschulschrift
  • Titel: Etablierung eines Verfahrens zum Nachweis epigenetischer Biomarker im peripheren Blut zur Stratifizierung der Therapie des Rektumkarzinoms
  • Beteiligte: Thormann, Tobias [VerfasserIn]; Legler, Tobias [Betreuer]; Legler, Tobias [Gutachter]; Gaedcke, Jochen [Gutachter]
  • Erschienen: Göttingen, 2015
  • Weitere Titel: Nebentitel: Fully-automated hypermethylation testing by One-Step-Real-Time-PCR of 6 different potential epigenetic biomarkers in peripheral blood for rectal cancer detection and follow-up.
  • Umfang: 1 Online-Ressource
  • Sprache: Deutsch
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  • Schlagwörter: Hochschulschrift
  • Beschreibung: Bisulfite treatment based assays are mainly used to detect aberrant, tumor specific methylation of specific DNA regions either from tissue or from free circulating DNA in plasma or serum. Since bisulfate based protocols are difficult to fully automate the aim of this study was to develop a rapid protocol which allows the detection and follow-up of rectal cancer from the primary blood tube. Methods: In an one step methylation sensitive, restriction enzyme based quantitative Real-Time PCR, hypermethylation in the promoter regions of RUNX3, NEUROG1, SEPT9, HLTF, TMEFF2, SDC2 and RASSF1A was in...
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  • Zugangsstatus: Freier Zugang