Erschienen in:
EPJ Web of Conferences, 266 (2022), Seite 04004
Sprache:
Ohne Angabe
DOI:
10.1051/epjconf/202226604004
ISSN:
2100-014X
Entstehung:
Anmerkungen:
Beschreibung:
Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets along an inclined tube for applications in flow-driven imaging. The element, which is based on a multi-focal Fresnel zone plate and a linear grating, generates in combination with a refractive cylindrical lens five thin light sheets of equal intensity.