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Nakagaki, Wilson Romero;
Camilli, José Angelo
Spontaneous Healing Capacity of Calvarial Bone Defects in mdx Mice
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- Medientyp: E-Artikel
- Titel: Spontaneous Healing Capacity of Calvarial Bone Defects in mdx Mice
- Beteiligte: Nakagaki, Wilson Romero; Camilli, José Angelo
- Erschienen: Wiley, 2012
- Erschienen in: The Anatomical Record
- Umfang: 590-596
- Sprache: Englisch
- DOI: 10.1002/ar.22412
- ISSN: 1932-8486; 1932-8494
- Schlagwörter: Ecology, Evolution, Behavior and Systematics ; Histology ; Biotechnology ; Anatomy
- Zusammenfassung: <jats:title>Abstract</jats:title><jats:p>The <jats:italic>mdx</jats:italic> mouse is an experimental model widely used for the study of Duchenne muscular dystrophy, which is characterized by the lack of dystrophin and cycles of muscle degeneration/regeneration. Studies demonstrated elevated levels of growth factors and accelerated skin wound repair in these animals. We therefore raised the hypothesis that the bone repair process might also be altered in these animals. Thus, the objective of this study was to evaluate the spontaneous healing of calvarial defects in <jats:italic>mdx</jats:italic> mice by histomorphometric analysis. Animals (45 days old) were divided into <jats:italic>mdx</jats:italic> and control groups. A defect measuring 2 mm in diameter was produced surgically in the right parietal bone of each animal. The animals were sacrificed 15, 30, and 60 days after surgery, and the skulls were processed by routine histological procedures. No difference in the volume of new bone inside the defect was observed between the two groups at any of the three postoperative time points. There was also no difference between the different periods of healing when each group was analyzed separately. The lower quality of femoral and calvarial bone in <jats:italic>mdx</jats:italic> mice reported in previous studies and the similar bone regeneration rates seen in two groups suggest that the healing capacity of calvarial defects was more expressive in <jats:italic>mdx</jats:italic> mice than in control animals. An increase in the amount of osteogenic factors released by damaged myofibers may have favored osteogenesis during bone defect healing in <jats:italic>mdx</jats:italic> mice. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.</jats:p>
- Beschreibung: <jats:title>Abstract</jats:title><jats:p>The <jats:italic>mdx</jats:italic> mouse is an experimental model widely used for the study of Duchenne muscular dystrophy, which is characterized by the lack of dystrophin and cycles of muscle degeneration/regeneration. Studies demonstrated elevated levels of growth factors and accelerated skin wound repair in these animals. We therefore raised the hypothesis that the bone repair process might also be altered in these animals. Thus, the objective of this study was to evaluate the spontaneous healing of calvarial defects in <jats:italic>mdx</jats:italic> mice by histomorphometric analysis. Animals (45 days old) were divided into <jats:italic>mdx</jats:italic> and control groups. A defect measuring 2 mm in diameter was produced surgically in the right parietal bone of each animal. The animals were sacrificed 15, 30, and 60 days after surgery, and the skulls were processed by routine histological procedures. No difference in the volume of new bone inside the defect was observed between the two groups at any of the three postoperative time points. There was also no difference between the different periods of healing when each group was analyzed separately. The lower quality of femoral and calvarial bone in <jats:italic>mdx</jats:italic> mice reported in previous studies and the similar bone regeneration rates seen in two groups suggest that the healing capacity of calvarial defects was more expressive in <jats:italic>mdx</jats:italic> mice than in control animals. An increase in the amount of osteogenic factors released by damaged myofibers may have favored osteogenesis during bone defect healing in <jats:italic>mdx</jats:italic> mice. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.</jats:p>
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