McGrath, John W.;
Hammerschmidt, Friedrich;
Kählig, Hanspeter;
Wuggenig, Frank;
Lamprecht, Günther;
Quinn, John P.
Studies on the Biodegradation of Fosfomycin: Synthesis of 13C‐Labeled Intermediates, Feeding Experiments with Rhizobium huakuii PMY1, and Isolation of Labeled Amino Acids from Cell Mass by HPLC
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Medientyp:
E-Artikel
Titel:
Studies on the Biodegradation of Fosfomycin: Synthesis of 13C‐Labeled Intermediates, Feeding Experiments with Rhizobium huakuii PMY1, and Isolation of Labeled Amino Acids from Cell Mass by HPLC
Beteiligte:
McGrath, John W.;
Hammerschmidt, Friedrich;
Kählig, Hanspeter;
Wuggenig, Frank;
Lamprecht, Günther;
Quinn, John P.
Beschreibung:
<jats:title>Abstract</jats:title><jats:p>Racemic (1<jats:italic>R*</jats:italic>,2<jats:italic>R*</jats:italic>)‐1,2‐dihydroxy‐[1‐<jats:sup>13</jats:sup>C<jats:sub>1</jats:sub>]propylphosphonic acid and 1‐hydroxy‐[1‐<jats:sup>13</jats:sup>C<jats:sub>1</jats:sub>]acetone were synthesized and fed to <jats:italic>R. huakuii</jats:italic> PMY1. Alanine and a mixture of valine and methionine were isolated as their <jats:italic>N</jats:italic>‐acetyl derivatives from the cell hydrolysate by reversed‐phase HPLC and analyzed by NMR spectroscopy. It was found that the carbon atoms of the respective carboxyl groups were highly <jats:sup>13</jats:sup>C‐labeled (up to 65 %). Hydroxyacetone is therefore considered an obligatory intermediate of the biodegradation of fosfomycin by <jats:italic>R. huakuii</jats:italic> PMY1.</jats:p>