Zelenin, Alexander V.;
Poletaev, Andrey I.;
Stepanova, Natalija G.;
Barsky, Viktor E.;
Kolesnikov, Viktor A.;
Nikitin, Sergei M.;
Zhuze, Alexey L.;
Gnutchev, Nikolai V.
7‐amino‐actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry
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Medientyp:
E-Artikel
Titel:
7‐amino‐actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry
Beteiligte:
Zelenin, Alexander V.;
Poletaev, Andrey I.;
Stepanova, Natalija G.;
Barsky, Viktor E.;
Kolesnikov, Viktor A.;
Nikitin, Sergei M.;
Zhuze, Alexey L.;
Gnutchev, Nikolai V.
Zusammenfassung:
<jats:title>Abstract</jats:title><jats:p>A technique for DNA amount determination by flow cytometry based on the use of 7‐amino‐actinomycin D (7‐amino‐AMD), a fluorescent analogue of antibiotic actinomycin has been investigated, and a particular staining procedure has been developed. The procedure includes short fixation in 70% ethanol and staining for 20 min in 10‐5M solution of 7‐amino‐AMD at pH7. The results of DNA content measurements are very reproducible. The histograms obtained have a coefficient of variation less than 3%.</jats:p><jats:p>The absorption maximum of the complex of 7‐amino‐AMD with DNA is situated in the green spectrum region, making this stain particularly suitable for argon laser flow cytometry.</jats:p>
Beschreibung:
<jats:title>Abstract</jats:title><jats:p>A technique for DNA amount determination by flow cytometry based on the use of 7‐amino‐actinomycin D (7‐amino‐AMD), a fluorescent analogue of antibiotic actinomycin has been investigated, and a particular staining procedure has been developed. The procedure includes short fixation in 70% ethanol and staining for 20 min in 10‐5M solution of 7‐amino‐AMD at pH7. The results of DNA content measurements are very reproducible. The histograms obtained have a coefficient of variation less than 3%.</jats:p><jats:p>The absorption maximum of the complex of 7‐amino‐AMD with DNA is situated in the green spectrum region, making this stain particularly suitable for argon laser flow cytometry.</jats:p>