• Medientyp: E-Artikel
  • Titel: 7‐amino‐actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry
  • Beteiligte: Zelenin, Alexander V.; Poletaev, Andrey I.; Stepanova, Natalija G.; Barsky, Viktor E.; Kolesnikov, Viktor A.; Nikitin, Sergei M.; Zhuze, Alexey L.; Gnutchev, Nikolai V.
  • Erschienen: Wiley, 1984
  • Erschienen in: Cytometry
  • Umfang: 348-354
  • Sprache: Englisch
  • DOI: 10.1002/cyto.990050410
  • ISSN: 0196-4763; 1097-0320
  • Schlagwörter: Cell Biology ; Endocrinology ; Hematology ; Biophysics ; Pathology and Forensic Medicine
  • Zusammenfassung: <jats:title>Abstract</jats:title><jats:p>A technique for DNA amount determination by flow cytometry based on the use of 7‐amino‐actinomycin D (7‐amino‐AMD), a fluorescent analogue of antibiotic actinomycin has been investigated, and a particular staining procedure has been developed. The procedure includes short fixation in 70% ethanol and staining for 20 min in 10‐5M solution of 7‐amino‐AMD at pH7. The results of DNA content measurements are very reproducible. The histograms obtained have a coefficient of variation less than 3%.</jats:p><jats:p>The absorption maximum of the complex of 7‐amino‐AMD with DNA is situated in the green spectrum region, making this stain particularly suitable for argon laser flow cytometry.</jats:p>
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>A technique for DNA amount determination by flow cytometry based on the use of 7‐amino‐actinomycin D (7‐amino‐AMD), a fluorescent analogue of antibiotic actinomycin has been investigated, and a particular staining procedure has been developed. The procedure includes short fixation in 70% ethanol and staining for 20 min in 10‐5M solution of 7‐amino‐AMD at pH7. The results of DNA content measurements are very reproducible. The histograms obtained have a coefficient of variation less than 3%.</jats:p><jats:p>The absorption maximum of the complex of 7‐amino‐AMD with DNA is situated in the green spectrum region, making this stain particularly suitable for argon laser flow cytometry.</jats:p>
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