• Medientyp: E-Artikel
  • Titel: Combined comparative genomic hybridization and genomic microarray for detection of gene amplifications in pulmonary artery intimal sarcomas and adrenocortical tumors
  • Beteiligte: Zhao, Jianming; Roth, Jürgen; Bode‐Lesniewska, Beata; Pfaltz, Madeleine; Heitz, Philipp U.; Komminoth, Paul
  • Erschienen: Wiley, 2002
  • Erschienen in: Genes, Chromosomes and Cancer
  • Umfang: 48-57
  • Sprache: Englisch
  • DOI: 10.1002/gcc.10035
  • ISSN: 1045-2257; 1098-2264
  • Schlagwörter: Cancer Research ; Genetics
  • Zusammenfassung: <jats:title>Abstract</jats:title><jats:p>Identification of gene amplifications in human tumors is important for the understanding of tumorigenesis and may lead to discovery of diagnostic and prognostic markers. In this study, we used a microarray‐based comparative genomic hybridization (CGH) technique, combined with conventional CGH, to identify gene amplifications in 43 tumors including eight pulmonary artery intimal sarcomas and 35 adrenocortical tumors. Conventional CGH revealed gains or amplifications of 12q13–q15 in six sarcomas and in two adrenocortical carcinomas. Using microarrays, we demonstrated that, among genes located on 12q13–q15, <jats:italic>SAS/CDK4</jats:italic> were amplified in six sarcomas, and <jats:italic>MDM2</jats:italic> and <jats:italic>GLI</jats:italic> in five and four sarcomas, respectively. The two adrenocortical tumors showed coamplifications of <jats:italic>SAS/CDK4</jats:italic> and <jats:italic>MDM2.</jats:italic> Furthermore, PDGFRA (located on 4q12) amplification was identified in five sarcomas. Our data demonstrate: (1) amplifications of <jats:italic>SAS/CDK4, MDM2, GLI,</jats:italic> and <jats:italic>PDGFRA</jats:italic> are strongly associated with the tumorigenesis of pulmonary artery intimal sarcomas, whereas <jats:italic>SAS/CDK4</jats:italic> and <jats:italic>MDM2</jats:italic> coamplification may contribute to the progression of adrenocortical tumors; (2) microarray‐based CGH is a useful tool for simultaneous detection of multiple gene amplifications, with a high sensitivity and resolution compared to that of conventional CGH. © 2002 Wiley‐Liss, Inc.</jats:p>
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>Identification of gene amplifications in human tumors is important for the understanding of tumorigenesis and may lead to discovery of diagnostic and prognostic markers. In this study, we used a microarray‐based comparative genomic hybridization (CGH) technique, combined with conventional CGH, to identify gene amplifications in 43 tumors including eight pulmonary artery intimal sarcomas and 35 adrenocortical tumors. Conventional CGH revealed gains or amplifications of 12q13–q15 in six sarcomas and in two adrenocortical carcinomas. Using microarrays, we demonstrated that, among genes located on 12q13–q15, <jats:italic>SAS/CDK4</jats:italic> were amplified in six sarcomas, and <jats:italic>MDM2</jats:italic> and <jats:italic>GLI</jats:italic> in five and four sarcomas, respectively. The two adrenocortical tumors showed coamplifications of <jats:italic>SAS/CDK4</jats:italic> and <jats:italic>MDM2.</jats:italic> Furthermore, PDGFRA (located on 4q12) amplification was identified in five sarcomas. Our data demonstrate: (1) amplifications of <jats:italic>SAS/CDK4, MDM2, GLI,</jats:italic> and <jats:italic>PDGFRA</jats:italic> are strongly associated with the tumorigenesis of pulmonary artery intimal sarcomas, whereas <jats:italic>SAS/CDK4</jats:italic> and <jats:italic>MDM2</jats:italic> coamplification may contribute to the progression of adrenocortical tumors; (2) microarray‐based CGH is a useful tool for simultaneous detection of multiple gene amplifications, with a high sensitivity and resolution compared to that of conventional CGH. © 2002 Wiley‐Liss, Inc.</jats:p>
  • Anmerkungen: