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Medientyp: E-Artikel Titel: Expression profiling of CC531 colon carcinoma cells reveals similar regulation of β‐catenin target genes by both butyrate and aspirin Beteiligte: Germann, Anja; Dihlmann, Susanne; Hergenhahn, Manfred; von Knebel Doeberitz, Magnus; Koesters, Robert Erschienen: Wiley, 2003 Erschienen in: International Journal of Cancer Sprache: Englisch DOI: 10.1002/ijc.11215 ISSN: 0020-7136; 1097-0215 Schlagwörter: Cancer Research ; Oncology Entstehung: Anmerkungen: Beschreibung: <jats:title>Abstract</jats:title><jats:p>The CC531 cell line has been widely used to study different aspects of tumor growth and metastasis and provides an excellent experimental platform to develop novel antitumor strategies. To characterize the CC531 model at the molecular level, we screened for mutations in genes covering important signal‐transduction pathways that are known to play major roles during colon carcinogenesis, the wnt and the ki‐ras signaling pathways. We found both a prototypic β‐<jats:italic>catenin</jats:italic> (<jats:italic>Ctnnb1</jats:italic>) mutation (Thr<jats:sup>41</jats:sup>Ile) and a <jats:italic>ki</jats:italic>‐<jats:italic>ras</jats:italic> (G12D) mutation, providing unambiguous evidence for the constitutive activation of these pathways in CC531 cells. We further established comprehensive gene expression profiles of CC531 cells and investigated the molecular response to 2 antitumor drugs, butyrate and aspirin. Using oligonucleotide microarrays, we screened the expression levels of 7,700 genes and identified a total of 398 genes whose expression was significantly changed upon treatment with butyrate. When using aspirin, 121 genes were significantly altered. Interestingly, 36 genes were regulated by both butyrate and aspirin and 35 of them were regulated in the same direction. We found 7 differentially expressed genes, <jats:italic>cyclin D1, cyclin E, c‐myc, Fosl1, c‐fos, Cd44</jats:italic> and <jats:italic>follistatin</jats:italic>, which are known targets of the β‐catenin and/or the ras pathway. In all cases, butyrate and aspirin reversed the changes in expression normally found in response to active signaling of these oncogenic pathways. The microarray data are available (<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://ncbi.nlm.nih.gov/geo/">http://ncbi.nlm.nih.gov/geo/</jats:ext-link>). © 2003 Wiley‐Liss, Inc.</jats:p> Zugangsstatus: Freier Zugang