Restoration of down‐regulated PDGF receptors by TGF‐β in human embryonic fibroblasts : Enhanced response during cellular in vitro aging
: Enhanced response during cellular in vitro aging
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Medientyp:
E-Artikel
Titel:
Restoration of down‐regulated PDGF receptors by TGF‐β in human embryonic fibroblasts : Enhanced response during cellular in vitro aging
:
Enhanced response during cellular in vitro aging
Beschreibung:
<jats:p>The study of [<jats:sup>125</jats:sup>I]PDGF‐BB binding to normal human embryonic lung fibroblasts, quiescent when cultured at sparsity in the presence of minute concentrations of homologous PDS, reveals approximately 2 × 10<jats:sup>5</jats:sup> binding sites for PDGF per cell; this number significantly increases during prolonged quiescence of the culture. As late as 48 h after down‐regulation of PDGF receptors, the cells restore only partially their capacity to bind PDGF, with aged cells (above CPD 45) responding more rapidly and efficiently than younger ones. TGF‐β significantly enhances restoration of PDGF receptors and, in aged cells in particular, its presence results in total receptor recovery within 24 h, suggesting a concerted action of PDGF and TGF‐β regulating the proliferation of human fibroblasts in tissue regeneration.</jats:p>