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Medientyp:
E-Artikel
Titel:
Interaction of heparin with annexin V
Beteiligte:
Capila, Ishan;
VanderNoot, Victoria A;
Mealy, Tanya R;
Seaton, Barbara A;
Linhardt, Robert J
Erschienen:
Wiley, 1999
Erschienen in:
FEBS Letters, 446 (1999) 2-3, Seite 327-330
Sprache:
Englisch
DOI:
10.1016/s0014-5793(99)00245-8
ISSN:
0014-5793;
1873-3468
Entstehung:
Anmerkungen:
Beschreibung:
The energetics and kinetics of the interaction of heparin with the Ca+2 and phospholipid binding protein annexin V, was examined and the minimum oligosaccharide sequence within heparin that binds annexin V was identified. Affinity chromatography studies confirmed the Ca+2 dependence of this binding interaction. Analysis of the data obtained from surface plasmon resonance afforded a Kd of ∼21 nM for the interaction of annexin V with end‐chain immobilized heparin and a Kd of ∼49 nM for the interaction with end‐chain immobilized heparan sulfate. Isothermal titration calorimetry showed the minimum annexin V binding oligosaccharide sequence within heparin corresponds to an octasaccharide sequence. The Kd of a heparin octasaccharide binding to annexin V was ∼1 μM with a binding stoichiometry of 1:1.