• Medientyp: E-Artikel
  • Titel: Interaction of heparin with annexin V
  • Beteiligte: Capila, Ishan; VanderNoot, Victoria A; Mealy, Tanya R; Seaton, Barbara A; Linhardt, Robert J
  • Erschienen: Wiley, 1999
  • Erschienen in: FEBS Letters, 446 (1999) 2-3, Seite 327-330
  • Sprache: Englisch
  • DOI: 10.1016/s0014-5793(99)00245-8
  • ISSN: 0014-5793; 1873-3468
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: The energetics and kinetics of the interaction of heparin with the Ca+2 and phospholipid binding protein annexin V, was examined and the minimum oligosaccharide sequence within heparin that binds annexin V was identified. Affinity chromatography studies confirmed the Ca+2 dependence of this binding interaction. Analysis of the data obtained from surface plasmon resonance afforded a Kd of ∼21 nM for the interaction of annexin V with end‐chain immobilized heparin and a Kd of ∼49 nM for the interaction with end‐chain immobilized heparan sulfate. Isothermal titration calorimetry showed the minimum annexin V binding oligosaccharide sequence within heparin corresponds to an octasaccharide sequence. The Kd of a heparin octasaccharide binding to annexin V was ∼1 μM with a binding stoichiometry of 1:1.
  • Zugangsstatus: Freier Zugang