• Medientyp: E-Artikel
  • Titel: Fatty acid ketodienes and fatty acid ketotrienes: Michael addition acceptors that accumulate in wounded and diseased Arabidopsis leaves
  • Beteiligte: Vollenweider, Sabine; Weber, Hans; Stolz, Stephanie; Chételat, Aurore; Farmer, Edward E.
  • Erschienen: Wiley, 2000
  • Erschienen in: The Plant Journal
  • Umfang: 467-476
  • Sprache: Englisch
  • DOI: 10.1111/j.1365-313x.2000.00897.x
  • ISSN: 0960-7412; 1365-313X
  • Schlagwörter: Cell Biology ; Plant Science ; Genetics
  • Zusammenfassung: <jats:title>Summary</jats:title><jats:p>Physical damage and disease are known to lead to changes in the oxylipin signature of plants. We searched for oxylipins produced in response to both wounding and pathogenesis in <jats:italic>Arabidopsis</jats:italic> leaves. Linoleic acid 9‐ and 13‐ketodienes (KODEs) were found to accumulate in wounded leaves as well as in leaves infected with the pathogen <jats:italic>Pseudomonas syringae</jats:italic> pv. <jats:italic>tomato</jats:italic> (Pst). Quantification of the compounds showed that they accumulated to higher levels during the hypersensitive response to Pst <jats:italic>avrRpm1</jats:italic> than during infection with a Pst strain lacking an avirulence gene. KODEs are Michael addition acceptors, containing a chemically reactive α,β‐unsaturated carbonyl group. When infiltrated into leaves, KODEs were found to induce expression of the <jats:italic>GST1</jats:italic> gene, but vital staining indicated that these compounds also damaged plant cells. Several molecules typical of lipid oxidation, including malonaldehyde, also contain the α,β‐unsaturated carbonyl reactivity feature, and, when delivered in a volatile form, powerfully induced the expression of <jats:italic>GST1</jats:italic>. The results draw attention to the potential physiological importance of naturally occurring Michael addition acceptors in plants. In particular, these compounds could act directly, or indirectly via cell damage, as powerful gene activators and might also contribute to host cell death.</jats:p>
  • Beschreibung: <jats:title>Summary</jats:title><jats:p>Physical damage and disease are known to lead to changes in the oxylipin signature of plants. We searched for oxylipins produced in response to both wounding and pathogenesis in <jats:italic>Arabidopsis</jats:italic> leaves. Linoleic acid 9‐ and 13‐ketodienes (KODEs) were found to accumulate in wounded leaves as well as in leaves infected with the pathogen <jats:italic>Pseudomonas syringae</jats:italic> pv. <jats:italic>tomato</jats:italic> (Pst). Quantification of the compounds showed that they accumulated to higher levels during the hypersensitive response to Pst <jats:italic>avrRpm1</jats:italic> than during infection with a Pst strain lacking an avirulence gene. KODEs are Michael addition acceptors, containing a chemically reactive α,β‐unsaturated carbonyl group. When infiltrated into leaves, KODEs were found to induce expression of the <jats:italic>GST1</jats:italic> gene, but vital staining indicated that these compounds also damaged plant cells. Several molecules typical of lipid oxidation, including malonaldehyde, also contain the α,β‐unsaturated carbonyl reactivity feature, and, when delivered in a volatile form, powerfully induced the expression of <jats:italic>GST1</jats:italic>. The results draw attention to the potential physiological importance of naturally occurring Michael addition acceptors in plants. In particular, these compounds could act directly, or indirectly via cell damage, as powerful gene activators and might also contribute to host cell death.</jats:p>
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  • Zugangsstatus: Freier Zugang