• Medientyp: E-Artikel
  • Titel: Expression of tomato salicylic acid (SA)‐responsive pathogenesis‐related genes in Mi‐1‐mediated and SA‐induced resistance to root‐knot nematodes
  • Beteiligte: Molinari, Sergio; Fanelli, Elena; Leonetti, Paola
  • Erschienen: Wiley, 2014
  • Erschienen in: Molecular Plant Pathology
  • Umfang: 255-264
  • Sprache: Englisch
  • DOI: 10.1111/mpp.12085
  • ISSN: 1464-6722; 1364-3703
  • Schlagwörter: Plant Science ; Soil Science ; Agronomy and Crop Science ; Molecular Biology
  • Zusammenfassung: <jats:title>Summary</jats:title><jats:p>The expression pattern of pathogenesis‐related genes <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic>, <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐2</jats:italic> and <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐5</jats:italic>, considered as markers for salicylic acid (<jats:styled-content style="fixed-case">SA</jats:styled-content>)‐dependent systemic acquired resistance (<jats:styled-content style="fixed-case">SAR</jats:styled-content>), was examined in the roots and shoots of tomato plants pre‐treated with <jats:styled-content style="fixed-case">SA</jats:styled-content> and subsequently infected with root‐knot nematodes (<jats:styled-content style="fixed-case">RKNs</jats:styled-content>) (<jats:italic><jats:styled-content style="fixed-case">M</jats:styled-content>eloidogyne incognita</jats:italic>). <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic> was up‐regulated in both roots and shoots of <jats:styled-content style="fixed-case">SA</jats:styled-content>‐treated plants, whereas the expression of <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐5</jats:italic> was enhanced only in roots. The over‐expression of <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic> in the whole plant occurred as soon as 1 day after <jats:styled-content style="fixed-case">SA</jats:styled-content> treatment. Up‐regulation of the <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic> gene was considered to be the main marker of <jats:styled-content style="fixed-case">SAR</jats:styled-content> elicitation. One day after treatment, plants were inoculated with active juveniles (<jats:styled-content style="fixed-case">J2</jats:styled-content>s) of <jats:italic><jats:styled-content style="fixed-case">M</jats:styled-content>. incognita</jats:italic>. The number of <jats:styled-content style="fixed-case">J2</jats:styled-content>s that entered the roots and started to develop was significantly lower in <jats:styled-content style="fixed-case">SA</jats:styled-content>‐treated than in untreated plants at 5 and 15 days after inoculation. The expression pattern of <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic>, <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐2</jats:italic> and <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐5</jats:italic> was also examined in the roots and shoots of susceptible and <jats:italic><jats:styled-content style="fixed-case">Mi</jats:styled-content>‐1‐</jats:italic>carrying resistant tomato plants infected by <jats:styled-content style="fixed-case">RKNs</jats:styled-content>. Nematode infection produced a down‐regulation of <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content></jats:italic> genes in both roots and shoots of <jats:styled-content style="fixed-case">SA</jats:styled-content>‐treated and untreated plants, and in roots of <jats:italic><jats:styled-content style="fixed-case">Mi</jats:styled-content>‐</jats:italic>carrying resistant plants. Moreover, in resistant infected plants, <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content></jats:italic> gene expression, in particular <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic> gene expression, was highly induced in shoots. Thus, nematode infection was demonstrated to elicit <jats:styled-content style="fixed-case">SAR</jats:styled-content> in shoots of resistant plants. The data presented in this study show that the repression of host defence <jats:styled-content style="fixed-case">SA</jats:styled-content> signalling is associated with the successful development of <jats:styled-content style="fixed-case">RKNs</jats:styled-content>, and that <jats:styled-content style="fixed-case">SA</jats:styled-content> exogenously added as a soil drench is able to trigger a <jats:styled-content style="fixed-case">SAR</jats:styled-content>‐like response to <jats:styled-content style="fixed-case">RKNs</jats:styled-content> in tomato.</jats:p>
  • Beschreibung: <jats:title>Summary</jats:title><jats:p>The expression pattern of pathogenesis‐related genes <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic>, <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐2</jats:italic> and <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐5</jats:italic>, considered as markers for salicylic acid (<jats:styled-content style="fixed-case">SA</jats:styled-content>)‐dependent systemic acquired resistance (<jats:styled-content style="fixed-case">SAR</jats:styled-content>), was examined in the roots and shoots of tomato plants pre‐treated with <jats:styled-content style="fixed-case">SA</jats:styled-content> and subsequently infected with root‐knot nematodes (<jats:styled-content style="fixed-case">RKNs</jats:styled-content>) (<jats:italic><jats:styled-content style="fixed-case">M</jats:styled-content>eloidogyne incognita</jats:italic>). <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic> was up‐regulated in both roots and shoots of <jats:styled-content style="fixed-case">SA</jats:styled-content>‐treated plants, whereas the expression of <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐5</jats:italic> was enhanced only in roots. The over‐expression of <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic> in the whole plant occurred as soon as 1 day after <jats:styled-content style="fixed-case">SA</jats:styled-content> treatment. Up‐regulation of the <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic> gene was considered to be the main marker of <jats:styled-content style="fixed-case">SAR</jats:styled-content> elicitation. One day after treatment, plants were inoculated with active juveniles (<jats:styled-content style="fixed-case">J2</jats:styled-content>s) of <jats:italic><jats:styled-content style="fixed-case">M</jats:styled-content>. incognita</jats:italic>. The number of <jats:styled-content style="fixed-case">J2</jats:styled-content>s that entered the roots and started to develop was significantly lower in <jats:styled-content style="fixed-case">SA</jats:styled-content>‐treated than in untreated plants at 5 and 15 days after inoculation. The expression pattern of <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic>, <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐2</jats:italic> and <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐5</jats:italic> was also examined in the roots and shoots of susceptible and <jats:italic><jats:styled-content style="fixed-case">Mi</jats:styled-content>‐1‐</jats:italic>carrying resistant tomato plants infected by <jats:styled-content style="fixed-case">RKNs</jats:styled-content>. Nematode infection produced a down‐regulation of <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content></jats:italic> genes in both roots and shoots of <jats:styled-content style="fixed-case">SA</jats:styled-content>‐treated and untreated plants, and in roots of <jats:italic><jats:styled-content style="fixed-case">Mi</jats:styled-content>‐</jats:italic>carrying resistant plants. Moreover, in resistant infected plants, <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content></jats:italic> gene expression, in particular <jats:italic><jats:styled-content style="fixed-case">PR</jats:styled-content>‐1</jats:italic> gene expression, was highly induced in shoots. Thus, nematode infection was demonstrated to elicit <jats:styled-content style="fixed-case">SAR</jats:styled-content> in shoots of resistant plants. The data presented in this study show that the repression of host defence <jats:styled-content style="fixed-case">SA</jats:styled-content> signalling is associated with the successful development of <jats:styled-content style="fixed-case">RKNs</jats:styled-content>, and that <jats:styled-content style="fixed-case">SA</jats:styled-content> exogenously added as a soil drench is able to trigger a <jats:styled-content style="fixed-case">SAR</jats:styled-content>‐like response to <jats:styled-content style="fixed-case">RKNs</jats:styled-content> in tomato.</jats:p>
  • Anmerkungen:
  • Zugangsstatus: Freier Zugang