• Medientyp: E-Artikel
  • Titel: Characterization of the Genome of the Dairy Lactobacillus helveticus Bacteriophage ΦAQ113
  • Beteiligte: Zago, Miriam; Scaltriti, Erika; Rossetti, Lia; Guffanti, Alessandro; Armiento, Angelarita; Fornasari, Maria Emanuela; Grolli, Stefano; Carminati, Domenico; Brini, Elena; Pavan, Paolo; Felsani, Armando; D'Urzo, Annalisa; Moles, Anna; Claude, Jean-Baptiste; Grandori, Rita; Ramoni, Roberto; Giraffa, Giorgio
  • Erschienen: American Society for Microbiology, 2013
  • Erschienen in: Applied and Environmental Microbiology
  • Sprache: Englisch
  • DOI: 10.1128/aem.00620-13
  • ISSN: 0099-2240; 1098-5336
  • Schlagwörter: Ecology ; Applied Microbiology and Biotechnology ; Food Science ; Biotechnology
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  • Beschreibung: <jats:title>ABSTRACT</jats:title> <jats:p> The complete genomic sequence of the dairy <jats:named-content content-type="genus-species">Lactobacillus helveticus</jats:named-content> bacteriophage ΦAQ113 was determined. Phage ΦAQ113 is a <jats:named-content content-type="genus-species">Myoviridae</jats:named-content> bacteriophage with an isometric capsid and a contractile tail. The final assembled consensus sequence revealed a linear, circularly permuted, double-stranded DNA genome with a size of 36,566 bp and a G+C content of 37%. Fifty-six open reading frames (ORFs) were predicted, and a putative function was assigned to approximately 90% of them. The ΦAQ113 genome shows functionally related genes clustered together in a genome structure composed of modules for DNA replication/regulation, DNA packaging, head and tail morphogenesis, cell lysis, and lysogeny. The identification of genes involved in the establishment of lysogeny indicates that it may have originated as a temperate phage, even if it was isolated from natural cheese whey starters as a virulent phage, because it is able to propagate in a sensitive host strain. Additionally, we discovered that the ΦAQ113 phage genome is closely related to <jats:named-content content-type="genus-species">Lactobacillus gasseri</jats:named-content> phage KC5a and <jats:named-content content-type="genus-species">Lactobacillus johnsonii</jats:named-content> phage Lj771 genomes. The phylogenetic similarities between <jats:named-content content-type="genus-species">L. helveticus</jats:named-content> phage ΦAQ113 and two phages that belong to gut species confirm a possible common ancestral origin and support the increasing consideration of <jats:named-content content-type="genus-species">L. helveticus</jats:named-content> as a health-promoting organism. </jats:p>
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