• Medientyp: E-Artikel
  • Titel: Injury-Induced Inflammation and Inadequate HSP Expression in Mesothelial Cells upon Repeat Exposure to Dual-Chamber Bag Peritoneal Dialysis Fluids
  • Beteiligte: Bender, Thorsten O.; Kratochwill, Klaus; Herzog, Rebecca; Ulbrich, Andrea; Böhm, Michael; Jörres, Achim; Aufricht, Christoph
  • Erschienen: SAGE Publications, 2015
  • Erschienen in: The International Journal of Artificial Organs
  • Umfang: 530-536
  • Sprache: Englisch
  • DOI: 10.5301/ijao.5000438
  • ISSN: 0391-3988; 1724-6040
  • Schlagwörter: Biomedical Engineering ; Biomaterials ; General Medicine ; Medicine (miscellaneous) ; Bioengineering
  • Zusammenfassung: <jats:sec><jats:title>Purpose</jats:title><jats:p>Peritoneal dialysis fluids (PDFs) may induce inadequate heat-shock protein (HSP) expression and injury-related inflammation in exposed mesothelial cells. The aim of this study was to relate cellular injury to these cellular responses in mesothelial cells following repeated exposure to 3 commercial PDFs with different biocompatibility profiles.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Primary cultures of human peritoneal mesothelial cells (HPMC) were exposed to a 1:2 mixture of cell culture medium and CAPD2 (single-chamber bag PDF; Fresenius, Bad Homburg, Germany), Physioneal (dual-chamber bag PDF; Baxter, Deerfield, IL, USA) or Balance (dual-chamber bag PDF, Fresenius) for up to 10 days exposure time (4 dwells). Supernatant was analyzed for LDH, IL-6, and IL-8, cells for HSP-72 expression, and protein content.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>PDF exposure resulted in a biphasic pattern of cell damage switching from an earlier phase with increased injury by single-chamber PDF to a delayed phase with increased susceptibility to dual-chamber PDF. Sterile inflammation was related to LDH release over time and could be reproduced by exposure to necrotic cellular material. PDF exposure resulted in low HSP-72 expression in all tested PDFs.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Exposure to single-chamber as well as to dual-chamber bag PDFs induce increased vulnerability of mesothelial cells to repeated exposure of the same solution. These effects were delayed with dual-chamber PDFs. Injury-induced inflammation and impaired HSP expression upon PDF exposure might initiate a vicious cycle with progredient mesothelial cell damage upon repeated PDF exposure. Certainly, interventional studies and translation of these results into the in vivo system is needed.</jats:p></jats:sec>
  • Beschreibung: <jats:sec><jats:title>Purpose</jats:title><jats:p>Peritoneal dialysis fluids (PDFs) may induce inadequate heat-shock protein (HSP) expression and injury-related inflammation in exposed mesothelial cells. The aim of this study was to relate cellular injury to these cellular responses in mesothelial cells following repeated exposure to 3 commercial PDFs with different biocompatibility profiles.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Primary cultures of human peritoneal mesothelial cells (HPMC) were exposed to a 1:2 mixture of cell culture medium and CAPD2 (single-chamber bag PDF; Fresenius, Bad Homburg, Germany), Physioneal (dual-chamber bag PDF; Baxter, Deerfield, IL, USA) or Balance (dual-chamber bag PDF, Fresenius) for up to 10 days exposure time (4 dwells). Supernatant was analyzed for LDH, IL-6, and IL-8, cells for HSP-72 expression, and protein content.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>PDF exposure resulted in a biphasic pattern of cell damage switching from an earlier phase with increased injury by single-chamber PDF to a delayed phase with increased susceptibility to dual-chamber PDF. Sterile inflammation was related to LDH release over time and could be reproduced by exposure to necrotic cellular material. PDF exposure resulted in low HSP-72 expression in all tested PDFs.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Exposure to single-chamber as well as to dual-chamber bag PDFs induce increased vulnerability of mesothelial cells to repeated exposure of the same solution. These effects were delayed with dual-chamber PDFs. Injury-induced inflammation and impaired HSP expression upon PDF exposure might initiate a vicious cycle with progredient mesothelial cell damage upon repeated PDF exposure. Certainly, interventional studies and translation of these results into the in vivo system is needed.</jats:p></jats:sec>
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