Burns,, James M.;
Shreffler, Wayne G.;
Benson, Darin R.;
Ghalib, Hashim W.;
Badaro, Roberto;
Reed, Steven G.
Molecular Characterization of a Kinesin-Related Antigen of Leishmania chagasi that Detects Specific Antibody in African and American Visceral Leishmaniasis
Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Molecular Characterization of a Kinesin-Related Antigen of Leishmania chagasi that Detects Specific Antibody in African and American Visceral Leishmaniasis
Beteiligte:
Burns,, James M.;
Shreffler, Wayne G.;
Benson, Darin R.;
Ghalib, Hashim W.;
Badaro, Roberto;
Reed, Steven G.
Erschienen:
National Academy of Sciences of the United States of America, 1993
Erschienen in:
Proceedings of the National Academy of Sciences of the United States of America, 90 (1993) 2, Seite 775-779
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
We report the cloning of a Leishmania chagasi antigen gene and an evaluation of leishmaniasis patient antibody responses to the recombinant protein, rK39. rK39 contains a 39-amino acid repeat that is part of a 230-kDa protein predominant in L. chagasi tissue amastigotes. Sequence analyses showed this protein, LcKin, to be related to the kinesin superfamily of metor proteins. Southern blot analyses demonstrated LcKin-related sequences in seven species of Leishmania, with conservation of the repeat between L. chagasi and Leishmania donovani. Serological evaluation revealed that 98% (56 of 57) of Brazilian and 100% (52 of 52) of Sudanese visceral leishmaniasis patients have high antibody levels to the rK39 repeat. Detectable anti-K39 antibody was virtually absent in cutaneous and mucosal leishmaniasis patients and in individuals infected with Trypanosoma cruzi. The data show that rK39 may replace crude parasite antigens as a basis for serological diagnosis of visceral leishmaniasis.