Voronina, Natalia
[Author]
;
Pfeifer, Alexander
[Other];
David, Robert
[Other];
Glocker, Michael
[Other]Universität Rostock,
Universität Rostock,
Universitätsmedizin Rostock,
Universitätsmedizin Rostock
Production and characterization of genetically modified magnetized cells for future targeted treatment of cardiovascular diseases
University thesis:
Dissertation, Universität Rostock, 2017
Footnote:
Description:
Gene therapy is a promising tool for cardiovascular disease treatment. Its wide clinical application is, however, limited by the lack of suitable targeted gene delivery systems. In this work, a non-viral vector was tested - composed by polyethyleneimine and magnetic nanoparticles (PEI/MNP) - with primary endothelial cells as in vitro model for angiogenesis and CD133+ hematopoietic stem cells. The obtained results indicate that transient genetic modification of cells with miRNA delivered by PEI/MNP is feasible and results in their magnetization sufficient for targeting and MRI detection.<eng>