University thesis:
Dissertation, Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald, 2019
Footnote:
Literaturverzeichnis: Seite 124-144
Tag der Promotion: 14.05.2019
Text auf Deutsch, Zusammenfassung auf Englisch
Description:
ASFV, Proteomanalyse, Virologie
African swine fever (ASP) is a virus disease, which affects members of the Suidae family such as warthogs, bush pigs, wild boars and domestic pigs. The virus is transmitted by direct contact between infected and naive animals, by ticks of the genus Ornithodoros or by contact with contaminated material. While the disease is generally asymptotic in warthogs and bush pigs, ASP causes high mortality among domestic swine and wild boar. Therefore, the recent spread of ASP in European countries poses a serious threat to pig farming in the EU. In addition, no effective treatment or vaccine is available to date. Literature on virus-host interactions which could be used as basis for antiviral strategies is scarce. the African swine fever virus (ASFV) is the only known member of the Asfarviridae family. The DNA genome of ASFV codes for over 150 genes. Knowledge about the expression products of individual genes is scarce and only a few proteins have been functionally characterized. The morphogenesis of ASFV is very complex. In addition to the doubly enveloped mature extracellular virion, intracellular virions carry only a single envelope, which complicates the preparation of pure extracellular virions. In previous in vitro studies, the composition of extracellular virus particles was analyzed by 2D gel electrophoresis. A purification protocol published in 1985 based on Percoll density gradient centrifugation and gel chromatography was used. The protocol was established for the ...