University thesis:
Dissertation, Georg-August-Universität Göttingen, 2019
Footnote:
Description:
In eukaryotes, pre-mRNA splicing is catalysed by the spliceosome, a highly complex and dynamic molecular machine, which assembles stepwise by the sequential recruitment of five small nuclear ribonucleoproteins (snRNPs) and numerous non-snRNP proteins. In humans, formation of the pre-catalytic, spliceosomal B complex with a stably associated tri-snRNP requires the action of the helicase Prp28, which displaces the U1 snRNP from the 5' splice site to be replaced by the U6 snRNA. At the same time, the so-called, B-specific proteins are recruited. Transformation of the B complex into an activate...