University thesis:
Dissertation, Universitätsmedizin der Universität Greifswald, 2019
Footnote:
Literaturverzeichnis: Seite 77-84
Zusammenfassung in deutscher Sprache
Description:
Keratin 15
Two principal stem cell pools orchestrate the fast cell turnover in the intestinal epithelium. Rapidly cycling Lgr5+ stem cells are intercalated between the Paneth cells at the crypt base (CBCs) and a putative slower cycling Bmi1+ cells are located at the +4 position above the crypt base. In the hair follicle and the esophageal epithelium, the intermediate filament Keratin 15 (Krt15) marks stem cells contributing to tissue repair. Herein, we demonstrated that Krt15 labels long-lived crypt cells harboring multipotency and self-renewing potential. Krt15+ crypt cells are resistant to high-dose radiation and contribute to crypt expansion following injury. These results suggest that Krt15 annotate a long-lived, multipotent crypt cell population harboring self-renewal capacity. Notably, Apc loss in Krt15+ cells lead to adenoma formation that can occasionally progress to adenocarcinoma.