University thesis:
Dissertation, Georg-August-Universität Göttingen, 2020
Footnote:
Description:
Since its first use as a genetically encoded fluorescent marker in 1994, GFP and its homologues have fundamentally revolutionized live-cell fluorescence imaging and became an essential tool for biomedical research. Many fluorescent proteins have been engineered from GFP-like proteins with excitation and emission covering the entirety of the visible spectrum. However, despite substantial efforts it was not possible to reach the near infrared (NIR) spectral region beyond 650nm with a GFP derived fluorescent protein. The NIR spectral region between 650 to 900nm is especially suited for live-ce...