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Media type:
E-Book;
Thesis
Title:
Evaluation and application of omics approaches to characterize molecular responses to abiotic stresses in plants
Other titles:
Übersetzung des Haupttitels: Evaluierung und Anwendung von Omics-Methoden zur Charakterisierung von abiotischem Stress in Pflanzen auf molekularer Ebene
University thesis:
Dissertation, Universität Potsdam, 2021
Footnote:
Description:
Due to global climate change providing food security for an increasing world population is a big challenge. Especially abiotic stressors have a strong negative effect on crop yield. To develop climate-adapted crops a comprehensive understanding of molecular alterations in the response of varying levels of environmental stresses is required. High throughput or ‘omics’ technologies can help to identify key-regulators and pathways of abiotic stress responses. In addition to obtain omics data also tools and statistical analyses need to be designed and evaluated to get reliable biological results. To address these issues, I have conducted three different studies covering two omics technologies. In the first study, I used transcriptomic data from the two polymorphic Arabidopsis thaliana accessions, namely Col-0 and N14, to evaluate seven computational tools for their ability to map and quantify Illumina single-end reads. Between 92% and 99% of the reads were mapped against the reference sequence. The raw count distributions obtained from ...