imprint:
[Erscheinungsort nicht ermittelbar]: [Verlag nicht ermittelbar], 2009
Language:
English
Identifier:
Origination:
University thesis:
Dissertation, 2009
Footnote:
Description:
Tese de doutoramento em Ciências (área de conhecimento em Física) ; The encapsulation of enzymes for optically addressed biosensor applications is an area of great importance. Firstly there is a need to provide a robust, optical quality host in which to incorporate the biomolecule. In principle the host matrix should be inert" (with minimum interaction with the guest enzyme), protective and should retain the enzyme within its structure, allowing maximum catalytic activity and free passage of the reactants both to and from the enzyme. Materials which are promising in this respect are silica glasses made via the sol-gel technique. They are optically transparent, have a high porosity and they template around the biomolecule providing a protective host. However, the encapsulation process can have adverse effects