Genome-Wide Identification of Lea Gene Family and Cold Response Mechanism of Bclea4-7 and Bclea4-18 in Non-Heading Chinese Cabbage [Brassica Campestris (Syn. Brassica Rapa) Ssp. Chinensis]
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E-Book
Title:
Genome-Wide Identification of Lea Gene Family and Cold Response Mechanism of Bclea4-7 and Bclea4-18 in Non-Heading Chinese Cabbage [Brassica Campestris (Syn. Brassica Rapa) Ssp. Chinensis]
Description:
Cold stress is a key factor limiting the yield and quality of non-heading Chinese cabbage. The hydrophilic protective protein LEA plays an important role in plant abiotic stress. In this study, 72 BcLEAs were identified from non-heading Chinese cabbage and divided into 9 subfamilies by phylogenetic analysis. Gene structure analysis showed that BcLEAs were unevenly distributed on 10 chromosomes, with few introns. Through analyzing the expression of these genes under cold stress by RNA-seq and qRT-PCR, two genes (BcLEA4-7 and BcLEA4-18) highly sensitive to cold stress were identified, whose roles in cold tolerance of non-heading Chinese cabbage were demonstrated by virus-induced gene silencing. The BcLEA promoters were analyzed to study the cold response mechanism of BcLEA4-7 and BcLEA4-18, revealing that both BcLEA4-7 and BcLEA4-18 promoters contained two CRT/DRE elements. Subsequently, it was found that the promoters isolated from non-heading Chinese cabbage could be activated at low temperatures. Further analysis showed BcCBF2 in non-heading Chinese cabbage interacted with two CRT/DRE elements in BcLEA4-7 and BcLEA4-18 promoters to stimulate their activity, indicating that BcCBF2 is an upstream regulator. Meanwhile, the CRT/DRE element located in BcLEA4-7 promoter (-219bp to -171bp) and BcLEA4-18 promoter (-234bp to -186bp) was more likely to be activated by BcCBF2, which may be attributed to its flanking sequence. These data laid a foundation for further understanding the functional role and regulatory mechanism of BcLEAs in cold stress tolerance