> Details

Torres-Olmos, Jorge Alejandro [Author]; Méndez-Gómez, Manuel [Author]; Castro-Mercado, Elda [Author]; Peña-Uribe, César Arturo [Author]; Campos-García, Jesús [Author]; López-Bucio, José [Author]; Reyes-de la Cruz, Homero [Author]; Garcia-Pineda, Ernesto [Author]

Hydrogen Peroxide Modulates Target of Rapamycin (Tor) in Arabidopsis Thaliana Seedlings to Control Growth and Development

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Book
  • Title: Hydrogen Peroxide Modulates Target of Rapamycin (Tor) in Arabidopsis Thaliana Seedlings to Control Growth and Development
  • Contributor: Torres-Olmos, Jorge Alejandro [VerfasserIn]; Méndez-Gómez, Manuel [VerfasserIn]; Castro-Mercado, Elda [VerfasserIn]; Peña-Uribe, César Arturo [VerfasserIn]; Campos-García, Jesús [VerfasserIn]; López-Bucio, José [VerfasserIn]; Reyes-de la Cruz, Homero [VerfasserIn]; Garcia-Pineda, Ernesto [VerfasserIn]
  • imprint: [S.l.]: SSRN, [2022]
  • Extent: 1 Online-Ressource (24 p)
  • Language: English
  • DOI: 10.2139/ssrn.4010650
  • Identifier:
  • Origination:
  • Footnote:
  • Description: Hydrogen peroxide (H 2 O 2 ) is an oxidizing agent that may affect biomolecules and cell integrity and represents an interesting molecule to investigate oxidative signaling in plant cells. Here, we assessed the interaction of H 2 O 2 with target of rapamycin (TOR), a serine/threonine protein kinase that plays a central role in controlling cell growth, size and metabolism in Arabidopsis thaliana . Through applying millimolar H 2 O 2 concentrations (0. 0.25, 0.5, 0.75 and 1 mM) to the growth medium of Arabidopsis seedlings, developmental traits such as primary root growth and lateral root formation could be correlated with the expression of cell cycle genes as well as detection of both the TOR protein and its biochemical activity on S6K. As the H 2 O 2 concentration increased, root length decreased in parallel with an increase in the number and density of lateral roots. The oxidizing agent promoted the development of leaf primordia with time, which inversely correlated with TOR expression. The TOR inhibitor AZD-8055 did not affect plant growth stimulated by H 2 O 2 treatment. The cell cycle reporter cyclin CYCB1;1::GUS was highly expressed with 1 mM H 2 O 2 and with 0.5 mM H 2 O 2 in combination with AZD-8055. Expression of the TOR::GUS reporter had two different responses to H 2 O 2 since 24 or 72 hours of treatment increased or decreased its expression, respectively. These effects were consistent with those seen for phosphorylation of the TOR target, S6K. The possible molecular mechanisms associated with the effects of H 2 O 2 on the cell cycle in roots and TOR expression and activity are discussed in the context of the results
  • Access State: Open Access