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Cruz-Magalhães, Valter [Author]; Nieto-Jacobo, Maria Fernanda [Author]; Rostás, Michael [Author]; Echaide-Aquino, Francisco Jesus [Author]; Esquivel-Naranjo, Ulises [Author]; Stewart, Alison [Author]; L. Loguercio, Leandro [Author]; Mendoza Mendoza, Artemio [Author]

Histidine Kinase Two-Component Response Regulators Ssk1, Skn7 and Rim15 Differentially Control Growth, Developmental and Volatile Organic Compounds Emissions as Stress Responses in Trichoderma Atroviride

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  • Media type: E-Book
  • Title: Histidine Kinase Two-Component Response Regulators Ssk1, Skn7 and Rim15 Differentially Control Growth, Developmental and Volatile Organic Compounds Emissions as Stress Responses in Trichoderma Atroviride
  • Contributor: Cruz-Magalhães, Valter [Author]; Nieto-Jacobo, Maria Fernanda [Author]; Rostás, Michael [Author]; Echaide-Aquino, Francisco Jesus [Author]; Esquivel-Naranjo, Ulises [Author]; Stewart, Alison [Author]; L. Loguercio, Leandro [Author]; Mendoza Mendoza, Artemio [Author]
  • Published: [S.l.]: SSRN, [2022]
  • Extent: 1 Online-Ressource (24 p)
  • Language: English
  • DOI: 10.2139/ssrn.4033312
  • Identifier:
  • Origination:
  • Footnote:
  • Description: The Skn7, Ssk1 and Rim15 proteins are response regulators involved in osmotic, oxidative and nutritional stress in fungi. In order to verify the involvement of these genes in growth, conidiation, direct antagonism against plant pathogens ( Rhizoctonia solani and Sclerotinia sclerotiorum ), production of volatile organic compounds (VOCs) with fungistatic effect, and interaction with plants (growth promotion), single mutants were generated, and the phenotypic patterns were analyzed in comparison to the Trichoderma atroviride IMI206040 wild-type ( wt ) strain. The mutants were submitted to osmotic, oxidative, membrane and cell wall stress conditions in vitro . The mutants Δ skn7 and Δ rim15 did not show significant differences at morphological level, nor was there a marked decrease in mycelial growth and conidiation in relation to wt , whereas Δ ssk1 had altered phenotypes in most conditions tested. The plant-growth promotion of Arabidopsis thaliana seedlings induced by VOCs was not modified by any of the mutants in relation to the wt strain, whereas the fungistatic activity was significantly altered in Δ ssk1 and Δ rim15 . The strain Δ ssk1 showed remarkable morphological differences, with decrease in mycelial growth, conidiation, and it was affected in the antagonistic capacity against plant pathogens. The impacts demonstrated by the deletion of ssk1 suggest this gene have a relevant participation in the signaling response to different stresses in T. atroviride , and in the interactive metabolism with phytopathogens and plants. On the other hand, unlike other fungal models, Skn7 did not appear to have a critical participation in the above-mentioned processes, and Rim15 seemed to confirm its involvement in modulating cellular responses to nutritional status, although with a possible cross-talk with other cellular processes. Our results suggest that Ssk1 likely plays a key regulatory role, not only in basic metabolisms of T. atroviride , but also in biocontrol-related characteristics
  • Access State: Open Access