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Frey, Dario [Author]; Bridson, Calum [Author]; Dittrich, A. Susanne [Author]; Gräber, Simon Y. [Author]; Stahl, Mirjam [Author]; Wege, Sabine [Author]; Herth, Felix [Author]; Sommerburg, Olaf [Author]; Schultz, Carsten [Author]; Dalpke, Alexander [Author]; Mall, Marcus A. [Author]; Boutin, Sébastien [Author]

Changes in microbiome dominance are associated with declining lung function and fluctuating inflammation in people with cystic fibrosis

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  • Media type: E-Article
  • Title: Changes in microbiome dominance are associated with declining lung function and fluctuating inflammation in people with cystic fibrosis
  • Contributor: Frey, Dario [Author]; Bridson, Calum [Author]; Dittrich, A. Susanne [Author]; Gräber, Simon Y. [Author]; Stahl, Mirjam [Author]; Wege, Sabine [Author]; Herth, Felix [Author]; Sommerburg, Olaf [Author]; Schultz, Carsten [Author]; Dalpke, Alexander [Author]; Mall, Marcus A. [Author]; Boutin, Sébastien [Author]
  • Published: 13 May 2022
  • Published in: Frontiers in microbiology ; 13(2022), Artikel-ID 885822, Seite 1-12
  • Language: English
  • DOI: 10.3389/fmicb.2022.885822
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  • Origination:
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  • Description: Airway inflammation and microbiome dysbiosis are hallmarks of cystic fibrosis (CF) lung disease. However, longitudinal studies are needed to decipher which factors contribute to the long-term evolution of these key features of CF. We therefore evaluated the relationship between fluctuation in microbiome and inflammatory parameters in a longitudinal study including a short- (1-year) and a long-term (3+ years) period. We collected 118 sputum samples from 26 CF adult patients and analyzed them by 16S rRNA gene sequencing. We measured the levels of inflammatory cytokines, neutrophil elastase, and anti-proteinases; lung function (FEV1% predicted); and BMI. The longitudinal evolution was analyzed based on (i) the rates of changes; (ii) the intra-patient stability of the variables; and (iii) the dependency of the rates of changes on the baseline values. We observed that the diversity of the microbiome was highly variable over a 1-year period, while the inflammatory markers showed a slower evolution, with significant changes only observed in the 3+ year cohort. Further, the degree of fluctuation of the biomass and the dominance of the microbiome were associated with changes in inflammatory markers, especially IL-1β and IL-8. This longitudinal study demonstrates for the first time that the long-term establishment and periodical variation of the abundance of a dominant pathogen is associated with a more severe increase in inflammation. This result indicates that a single time point or 1-year study might fail to reveal the correlation between microbial evolution and clinical degradation in cystic fibrosis.
  • Access State: Open Access