Giglio, Guilherme Lelis
[Author];
Alves, Alex Marquiti
[Author];
Cavalcante, Willame Araujo
[Author];
Takeda, Paula Yumi
[Author];
Paula, Carolina Tavares
[Author];
Pereira, Tiago Duarte Santos
[Author];
Damianovic, Márcia H. R. Z.
[Author]
Anammox Granular Biomass Adaptation in Warm Regions
: Evaluating its Feasibility for Transitioning from Sidestream to Mainstream Anaerobic Effluent Treatment
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Media type:
E-Book
Title:
Anammox Granular Biomass Adaptation in Warm Regions
:
Evaluating its Feasibility for Transitioning from Sidestream to Mainstream Anaerobic Effluent Treatment
Description:
Starting-up an anammox-based mainstream reactor by inoculating enriched sidestream-based biomass is a promising strategy for enhancing nitrogen removal in sewage treatment plants (STPs). However, implementation of anammox-based processes face challenges due to low temperatures and reduced nitrogen concentrations in mainstream wastewaters. Nonetheless, in warm regions, where anaerobic reactors have been successfully implemented in STPs, the mesophilic temperatures of wastewaters can facilitate the adoption of mainstream anammox. In this context, three bench-scale up-flow reactors (R1, R2, and R3) were used to assess the feasibility of applying mainstream anammox-based reactors in warm regions. To perform the anammox process, R1 and R2 were fed with synthetic medium composed with a nitrite/ammonium ratio (NO2--N/N-NH4+-N) of 1.32 and free from organic matter (OM). A portion of the anammox granular biomass, enriched for 607 days in R1 (sidestream), was transferred in R2 (mainstream) to treat synthetic domestic sewage with low nitrogen concentration under mesophilic temperatures. In R2, over 34 days, the nitrogen concentration was gradually decreased from 232 to 50 mgN.L-1, followed by a temperature reduction from 37 to 30 and finally to 25°C over 31 days. During the 65-day period, a high nitrogen removal efficiency (NRE) of 87.3 ± 4.7% was obtained in R2 with a nitrogen loading rate (NLR) ranging from 1.0 to 1.4 kgN.m-3.d-1. Adapted granular anammox biomass from R2 was used to start-up the R3. Nitrite supplementation was ceased to allow its production through automated intermittent aeration (AIA) and observe the occurrence of the partial nitritation-anammox (PNA) process in R3 (mainstream PNA). However, the adapted granular anammox biomass did not establish the PNA process by the AIA mode. Biomolecular analysis revealed that the biomass transferred from R2 to R3 had a high relative abundance of the anammox Candidatus Brocadia bacteria (29.4%), but no ammonium-oxidizing bacteria (AOB) were identified. When nitrite supplementation was reintroduced within AIA, the NRE reached 63% in R3, but no contribution of PNA was observed