Wong, John K. L.
[Author];
Jassowicz, Lena
[Author];
Herold-Mende, Christel
[Author];
Seiffert, Martina
[Author];
Mallm, Jan-Philipp
[Author];
Lichter, Peter
[Author];
Zapatka, Marc
[Author]
Description:
Here we present scSNPdemux, a sample demultiplexing pipeline for single-cell RNA sequencing data using natural genetic variations in humans. The pipeline requires alignment files from Cell Ranger (10× Genomics), a population SNP database and genotyped single nucleotide polymorphisms (SNPs) per sample. The tool works on sparse genotyping data in VCF format for sample identification.