In 2020, the 50 most common surgeries were performed a total of 15,823,464 times, which corresponds to approximately one operation for every five inhabitants in Germany. In each of these operations, hemostasis is a therapeutic requirement. Intraoperatively, electrocauterization is often used for this purpose, but this is associated with a risk of secondary bleeding, perforation, and tissue destruction. Cold physical plasma (gas plasma) can represent a new variant for hemorrhage management. This is an energetic gas that interacts through various mechanisms such as temperature, reactive chemical species, UV, and thermal radiation. In vitro studies were performed on human whole blood to identify a mechanism of action of gas plasma. In the present study, it was found that the majority of platelet activation by gas plasma (approx. 55 %) is due to hemolysis. The hemolysis was detected spectroscopically and quantified for different treatment times. The platelets respond with a PI3K/Akt/p38-mediated signaling cascade, which ultimately leads to their activation. Significance of intracellular ROS and hyperpolarization of platelet mitochondria was found during signal transduction. The signaling cascade can be explained by the influence of ADP on the P2Y12 receptor. An evaluation algorithm based on artificial intelligence was used, which demonstrated increased platelet aggregates after application of gas plasma. Gas plasma acts via a variety of reactive species and long-lived species such as hydrogen peroxide, hypochlorous acid and superoxide anions appear to contribute only slightly to the gas plasma effect. The explanation of hemolysis has been attributed to singlet oxygen and ozone. In addition, NO can act directly on platelets. The first measurement of the surface temperature of a liquid during treatment with gas plasma was carried out. A minor change in temperature was detected. Evaporation during gas plasma treatment was quantified. As the use of gas plasma accelerates the body's own coagulation mechanisms, has a disinfectant property and only few side effects are known, gas plasma has a high clinical potential in the field of surgical blood coagulation.