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Media type:
E-Book;
Thesis
Title:
AC electrokinetic immobilization of influenza viruses and antibodies on nanoelectrode arrays for on-chip immunoassays
Other titles:
Übersetzung des Haupttitels: AC elektrokinetische Immobilisierung von Influenzaviren und Antikörpern auf Nanoelektrodenarrays für on-Chip Immunoassays
University thesis:
Dissertation, Universität Potsdam, 2023
Footnote:
Description:
In the present thesis, AC electrokinetic forces, like dielectrophoresis and AC electroosmosis, were demonstrated as a simple and fast method to functionalize the surface of nanoelectrodes with submicrometer sized biological objects. These nanoelectrodes have a cylindrical shape with a diameter of 500 nm arranged in an array of 6256 electrodes. Due to its medical relevance influenza virus as well as anti-influenza antibodies were chosen as a model organism. Common methods to bring antibodies or proteins to biosensor surfaces are complex and time-consuming. In the present work, it was demonstrated that by applying AC electric fields influenza viruses and antibodies can be immobilized onto the nanoelectrodes within seconds without any prior chemical modification of neither the surface nor the immobilized biological object. The distribution of these immobilized objects is not uniform over the entire array, it exhibits a decreasing gradient from the outer row to the inner ones. Different causes for this gradient have been discussed, such ...