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Media type:
E-Book;
Thesis
Title:
Dissecting the role of the TusA protein for cell functionality and FtsZ ring assembly in Escherichia coli
Other titles:
Übersetzung des Haupttitels: Entschlüsselung der Rolle des TusA-Proteins für die Zellfunktionalität und FtsZ-Ringbildung in Escherichia coli
University thesis:
Dissertation, Universität Potsdam, 2023
Footnote:
Description:
In this work, the role of the TusA protein was investigated for the cell functionality and FtsZ ring assembly in Escherichia coli. TusA is the tRNA-2-thiouridine synthase that acts as a sulfur transferase in tRNA thiolation for the formation of 2-thiouridine at the position 34 (wobble base) of tRNALys, tRNAGlu and tRNAGln. It binds the persulfide form of sulfur and transfers it to further proteins during mnm5s2U tRNA modification at wobble position and for Moco biosynthesis. With this thiomodification of tRNA, the ribosome binding is more efficient and frameshifting is averted during the protein translation. Previous studies have revealed an essential role of TusA in bacterial cell physiology since deletion of the tusA gene resulted in retarded growth and filamentous cells during the exponential growth phase in a rich medium which suddenly disappeared during the stationary phase. This indicates a problem in the cell division process. Therefore the focus of this work was to investigate the role of TusA for cell functionality and FtsZ ring formation and thus the cell separation. [...]