Molecular embryology: methods and protocols

Media type: Book

Title: Molecular embryology : methods and protocols

Contains: pt. 1. The mouse embryoThe mouse as a developmental model / Paul T. Sharpe
Culture of postimplantation mouse embryos / Paul Martin and David L. Cockroft
Organ culture in the analysis of tissue interactions / Irma Thesleff and Carin Sahlberg
Treatment of mice with retinoids in vivo and in vitro : skeletal staining / Gillian M. Morriss-Kay
Cell grafting and labeling in postimplantation mouse embryos / Gabriel A. Quinlan, Paul A. Trainor, and Patrick P.L. Tam
Production of transgenic rodents by the microinjection of coloned DNA into fertilized one-celled eggs / San Ling Si-Hoe and David Murphy
Cre recombinase mediated alterations of the mouse genome using embryonic stem cells / Anna-Katerina Hadjantonakis, Melinda Pirity, and Andras Nagy
Gene trapping in mouse embryonic stem cells / Jane Brennan and William C. Skarnes
Production and growth of conditionally immortal cell lines from the H-2KbtsA58 transgenic mouse / Mark Noble
Reporter genes for the study of transcriptional regulation in transgenic mouse embryos / Jonathan D. Gilthorpe and Peter W.J. Rigby
Application of IacZ transgenic mice to cell lineage studies / Paul A. Trainor [and others]
Mouse primordial germ cells : isolation and in vitro culture / Patricia A. Labosky and Brigid L.M. Hogan
pt. 2. The chicken embryo ; The avian embryo: an overview / Ivor Mason
Chick embryos : incubation and isolation / Ivor Mason
New culture / Amata Hornbruch
Grafting Hensen's node / Claudio D. Stern
Grafting of Somites / Claudio D. Stern
Notochord grafts / Andrew Lumsden and Susanne Dietrich
Transplantation of avian neural tissue / Sarah Guthrie
Grafting of apical ridge and polarizing region / Cheryl Tickle
Tissue recombinations in collagen gels / Marysia Placzek and Kim Dale
Quail-chick chimeras / Marie-Aime Teillet, Catherine Ziller, and Nicole M. Le Douarin
Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo / Jonathan D.W. Clarke
pt. 3. Amphibian embryo ; An overview of Xenopus development / C. Michael Jones and Jim Smith
Mesoderm induction assays / C. Michael Jones and Jim Smith
Experimental embryological methods for analysis of neural induction in the amphibian / Ray Keller, Ann Poznanski, and Tamira Elul
A method for generating transgenic frog embryos / Enrique Amaya and Kristen L. Kroll
Axolotl/newt / Malcolm Maden
pt. 4. Zebrafish ; The Zebrafish : An Overview of Its Early Development / Nigel Holder and Qiling Xu
Small-Scale Marker-Based Screening For Mutations in Zebrafish Development / Peter D. Currie, Thomas F. Schilling, and Philip W. Ingham
Transgenic Zebrafish / Trevor Jowett
Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg For Analysis of Gene Function / Nigel Holder and Qiling Xu
Retinoids in Nonmammalian Embryos / Malcolm Maden
Part V. Nonvertebrate Chordates ; Protochordates / Peter W.H. Holland and Hiroshi Wada
Part VI. Retrovirus ; Gene Transfer to the Rodent Embryo by Retroviral Vectors / Grace K. Pavlath and Marla B. Luskin
Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses / Cairine Logan and Philippa Francis-West
Part VII. Molecular Techniques ; Subtractive Hybridization and Construction of cDNA Libraries / Bruce Blumberg and Juan Carlos Izpisua Belmonte
Differential Display of Eukaryotic mRNA / Antonio Tugores and Juan Carlos Izpisua Belmonte
RT-PCR on Embryos Using Degenerate Oligonucleotide Primers / Anthony Graham
Single-Cell RT-PCR cDNA Subtraction / Damian L. Weaver [and others]
In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections / Radma Mahmood and Ivor Mason
In Situ Hybridization to RNA in Whole Embryos / Huma Shamim, Radma Mahmood, and Ivor Mason
Wholemount In Situ Hybridization to Xenopus Embryos / C. Michael Jones and James C. Smith
Wholemount In Situ Hybridization to Amphioxus Embryo / Peter W.H. Holland
In Situ Hybridization to Sections (Nonradioactive) / Maria Rex and Paul J. Scotting
Immunohistochemistry Using Polyester Wax / Andrew Kent
Immunohistochemistry on Whole Embryos / Ivor Mason
Whole Embryo Assays for Programmed Cell Death / Anthony Graham
Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos : Optimal Ring and Roller-Bottle Culture Technique / Jonathan Cooke and Alison Isaac
Protein Techniques : Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting / David I. Jackson and Clive Dickson
Part VIII. Microscopy and Photography ; Microscopy and Photomicrography Techniques / Richard J.T. Wingate.

Contributor: Sharpe, Paul T. [Hrsg.]; Mason, Ivor [Other]

imprint: Totowa, NJ: Humana Press, 1999

Extent: XVI, 756 S.; Ill., graph. Darst; 24 cm

Language: English

ISBN: 0896033872

RVK notation: WC 4150 : Allgemeines, Praktika; "Methods of biochemical analysis"
WD 4150 : Molekularbiologie (hier nur einführende Werke, Lehrbücher, Übersichten über das Gesamtgebiet, sonst bei entsprechenden Systemstellen, z.B. Zellbiologie WE, Molekulargenetik WG 1700 ff.)

Keywords: Wirbeltiere > Embryologie > Methode

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Footnote: Literaturangaben

Molecular embryology : methods and protocols

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