Studien zur Biosynthese des fungalen Sekundärmetaboliten Ampullosporin und Untersuchung der Kultivierungsbedingungen des Produzenten Sepedonium ampullosporum
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Thesis
Title:
Studien zur Biosynthese des fungalen Sekundärmetaboliten Ampullosporin und Untersuchung der Kultivierungsbedingungen des Produzenten Sepedonium ampullosporum
Description:
At the present study cognitions on biosynthesis of the fungal, neuroleptic and peptaibol type peptide Ampullosporin were obtained. Two high molecular weight, strong with each other associated proteins of about 1.5 MDa and 350 kDa were detected as the putative ampullosporin synthetases, the 15-modular NRPS-system for the production of the 15-membered Ampullosporin. They were partially purified and biochemically characterized. By a later proof of in-vitro activity of Aib by partially purified protein extracts the NRPS-system was confirmed as a peptaibol synthetase. Moreover a gene fragment of about 2 kb showing highest homologies to the glutamin activating modules encoded by tex1 from Trichoderma virens could be amplified from the genome of Ampullosporin producing organism Sepedonium ampullosporum. Furtermore a standardised submerged cultivation procedure in shaking flasks with increased Ampullosporin formation up to 33 mg/l during reduced fermentation times was established. Monospecific antibodies against Ampullosporin were made and used for the localisation of this secondary metabolite in the producing organism by immunoelectron microscopy.