Description:
Binding reactions can be ideally investigated by Dual-Color Fluorescence Cross-Correlation Spectroscopy (FCCS). Here, this method is used to study the recruitment and dissociation of proteins to and from the spliceosome. The spliceosome is the cellular machinery responsible for removing non-coding introns from precursor mRNA (pre-mRNA). The spliceosome assembles on a pre-mRNA, which consists of two exons and one intron, stepwise by the binding of five snRNPs and several other proteins. This leads to the formation of the B complex which has no active catalytic center yet. Structural rearrang...