Description:
The aim of this study was to map humoral immune responses in rhesus macaques (RM) which had either been immunized and/or infected with different viruses. This was achieved by screening serum samples using peptide microarrays. Further goals were the optimization of the analysis and the minimization of the required sample volumes. For the readout, an independent computer-assisted algorithm was formulated which provided an objective method to optimize peptide microarray production and data mining. In order to screen large numbers of serum samples, new tools were developed that allowed a safe h...