Schade, Anna
[Author]
;
Steinhoff, Gustav
[Other];
Pützer, Brigitte Maria
[Other];
Brunner, Stefan
[Other]Universität Rostock,
Universitätsmedizin Rostock
MicroRNA-based stem cell modifications using magnetic transfection complexes
University thesis:
Dissertation, Universität Rostock, 2015
Footnote:
Description:
Regenerative properties of human mesenchymal stem cells (hMSC) can be enhanced by genetic modifications. Thus we aimed to develop a magnetic non-viral vector for microRNA (miR) delivery in freshly isolated hMSCs. miR/PEI/MNP transfection showed a better long term performance compared to miR/PEI transfection. Moreover magnetically modified hMSCs could be guided by an external magnet contributing to enhanced cell engraftment. Conclusively a magnetic non-viral carrier for efficient miR delivery in freshly isolated hMSCs was developed allowing specific control of stem cells and magnetic targeting.<eng>